Cancer cell invasion and EMT marker Expression - a three-dimensional study of the human cancer-host interface

P. Bronsert; K. Enderle-Ammour; M. Bader; S. Timme; M. Kuehs; A. Csanadi; G. Kayser; I. Kohler; D. Bausch; J. Hoeppner; U. T. Hopt; T. Keck; E. Stickeler; B. Passlick; O. Schilling; C. P. Reiss; Y. Vashist; T. Brabletz; J. Berger; J. Lotz; J. Olesch; M. Werner; U. F. Wellner

doi:10.1002/path.4416

Cancer cell invasion and EMT marker Expression - a three-dimensional study of the human cancer-host interface

P. Bronsert, K. Enderle-Ammour, M. Bader, S. Timme, M. Kuehs, A. Csanadi, G. Kayser, I. Kohler, D. Bausch, J. Hoeppner, U. T. Hopt, T. Keck, E. Stickeler, B. Passlick, O. Schilling, C. P. Reiss, Y. Vashist, T. Brabletz, J. Berger, J. LotzJ. Olesch, M. Werner, U. F. Wellner

University of Freiburg

57 Citations (Scopus)

Abstract

Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression.

Original language	English
Journal	The Journal of pathology
Volume	234
Issue number	3
Pages (from-to)	410-422
Number of pages	13
ISSN	0002-9440
DOIs	https://doi.org/10.1002/path.4416
Publication status	Published - 01.11.2014

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10.1002/path.4416

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Bronsert, P., Enderle-Ammour, K., Bader, M., Timme, S., Kuehs, M., Csanadi, A., Kayser, G., Kohler, I., Bausch, D., Hoeppner, J., Hopt, U. T., Keck, T., Stickeler, E., Passlick, B., Schilling, O., Reiss, C. P., Vashist, Y., Brabletz, T., Berger, J., ... Wellner, U. F. (2014). Cancer cell invasion and EMT marker Expression - a three-dimensional study of the human cancer-host interface. The Journal of pathology, 234(3), 410-422. https://doi.org/10.1002/path.4416

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title = "Cancer cell invasion and EMT marker Expression - a three-dimensional study of the human cancer-host interface",

abstract = "Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression.",

author = "P. Bronsert and K. Enderle-Ammour and M. Bader and S. Timme and M. Kuehs and A. Csanadi and G. Kayser and I. Kohler and D. Bausch and J. Hoeppner and Hopt, {U. T.} and T. Keck and E. Stickeler and B. Passlick and O. Schilling and Reiss, {C. P.} and Y. Vashist and T. Brabletz and J. Berger and J. Lotz and J. Olesch and M. Werner and Wellner, {U. F.}",

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Bronsert, P, Enderle-Ammour, K, Bader, M, Timme, S, Kuehs, M, Csanadi, A, Kayser, G, Kohler, I, Bausch, D, Hoeppner, J, Hopt, UT, Keck, T, Stickeler, E, Passlick, B, Schilling, O, Reiss, CP, Vashist, Y, Brabletz, T, Berger, J, Lotz, J, Olesch, J, Werner, M & Wellner, UF 2014, 'Cancer cell invasion and EMT marker Expression - a three-dimensional study of the human cancer-host interface', The Journal of pathology, vol. 234, no. 3, pp. 410-422. https://doi.org/10.1002/path.4416

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T1 - Cancer cell invasion and EMT marker Expression - a three-dimensional study of the human cancer-host interface

AU - Bronsert, P.

AU - Enderle-Ammour, K.

AU - Bader, M.

AU - Timme, S.

AU - Kuehs, M.

AU - Csanadi, A.

AU - Kayser, G.

AU - Kohler, I.

AU - Bausch, D.

AU - Hoeppner, J.

AU - Hopt, U. T.

AU - Keck, T.

AU - Stickeler, E.

AU - Passlick, B.

AU - Schilling, O.

AU - Reiss, C. P.

AU - Vashist, Y.

AU - Brabletz, T.

AU - Berger, J.

AU - Lotz, J.

AU - Olesch, J.

AU - Werner, M.

AU - Wellner, U. F.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression.

AB - Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression.

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JO - The Journal of pathology

JF - The Journal of pathology

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Cancer cell invasion and EMT marker Expression - a three-dimensional study of the human cancer-host interface

Abstract

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Epithelio-mesenchymal transition in invasion and metastasis of periampullary carcinoma

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Cancer cell invasion and EMT marker Expression - a three-dimensional study of the human cancer-host interface

Abstract

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Epithelio-mesenchymal transition in invasion and metastasis of periampullary carcinoma

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