Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells

Ayşegül Tura, Aizhan Alt, Julia Lüke, Salvatore Grisanti, Christos Haritoglou, Carsten H Meyer, Khaled Nassar, Matthias Lüke

Abstract

PURPOSE: To examine the viability and differentiation of retinal pigment epithelial (RPE) cells after exposure to the vital dye Acid Violet-17 (AV-17).

METHODS: Bovine RPE cells were incubated with AV-17 (0.0625-0.5 mg/mL) for 30 seconds or 5 minutes. Viability was determined by live/dead staining, cleaved CASP3 immunostainings, and MTT test. Actin cytoskeleton was visualized by Alexa 488-phalloidin. Immunocytochemistry was performed to determine the levels of ZO-1, CTNNB1, and KRT19.

RESULTS: Exposure to AV-17 at the concentrations of 0.25-0.5 mg/mL resulted in a dose-dependent decrease in viability, the loss of ZO-1 from tight junctions, translocation of CTNNB1 into the cytoplasm and nucleus, disarrangement of the actin cytoskeleton, and a slight increase in KRT19.

CONCLUSION: AV-17 at a concentration <0.125 mg/mL is likely to be well tolerated by the RPE cells, whereas the concentrations from 0.25 mg/mL onward can reduce viability and induce dedifferentiation particularly after long-term exposure.

Translated title of the contributionBiokompatibilität des Vitalfarbstoffs Acid Violet-17 auf retinalen Pigmentepithelzellen
Original languageEnglish
JournalClinical ophthalmology (Auckland, N.Z.)
Volume10
Pages (from-to)1435-45
Number of pages11
ISSN1177-5467
DOIs
Publication statusPublished - 2016

Fingerprint

Dive into the research topics of 'Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells'. Together they form a unique fingerprint.

Cite this