TY - JOUR
T1 - Assignment of the orphan nuclear receptor Nurr1 by NMR
AU - Michiels, Paul
AU - Atkins, Karen
AU - Ludwig, Christian
AU - Whittaker, Sara
AU - Van Dongen, Maria
AU - Günther, Ulrich
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/4
Y1 - 2010/4
N2 - The orphan nuclear receptor Nurr1 has been implicated in a number of conditions including Parkinson's disease and Schizophrenia. As such, it is of interest to study its interactions with other proteins, possibly mediated by small molecules, considering possible use as a drug target. We produced 2H, 15N, 13C labelled-Nurr1 to generate the backbone amide NH, carbonyl C′, Calpha and Cbeta assignments. About 84.0% of residues could be assigned. Most of the 37 missing assignments fall in 3 regions of the protein. Two of these surround a putative ligand-binding region of Nurr1, suggesting that this region of the protein is flexible, despite the ligand-binding pocket being filled with hydrophobic side-chains from residues surrounding the ligand binding pocket.
AB - The orphan nuclear receptor Nurr1 has been implicated in a number of conditions including Parkinson's disease and Schizophrenia. As such, it is of interest to study its interactions with other proteins, possibly mediated by small molecules, considering possible use as a drug target. We produced 2H, 15N, 13C labelled-Nurr1 to generate the backbone amide NH, carbonyl C′, Calpha and Cbeta assignments. About 84.0% of residues could be assigned. Most of the 37 missing assignments fall in 3 regions of the protein. Two of these surround a putative ligand-binding region of Nurr1, suggesting that this region of the protein is flexible, despite the ligand-binding pocket being filled with hydrophobic side-chains from residues surrounding the ligand binding pocket.
UR - http://www.scopus.com/inward/record.url?scp=77951808899&partnerID=8YFLogxK
U2 - 10.1007/s12104-010-9210-4
DO - 10.1007/s12104-010-9210-4
M3 - Journal articles
C2 - 20300892
AN - SCOPUS:77951808899
SN - 1874-2718
VL - 4
SP - 101
EP - 105
JO - Biomolecular NMR Assignments
JF - Biomolecular NMR Assignments
IS - 1
ER -