ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat

Ulrike Rein, Uwe Andag, Rainer Duden, Hans Dieter Schmitt, Anne Spang*

*Corresponding author for this work
96 Citations (Scopus)

Abstract

In eukaryotic cells, secretion is achieved by vesicular transport. Fusion of such vesicles with the correct target compartment relies on SNARE proteins on both vesicle (v-SNARE) and the target membranes (t-SNARE). At present it is not clear how v-SNAREs are incorporated into transport vesicles. Here, we show that binding of ADP-ribosylation factor (ARF)-GTPase-activating protein (GAP) to ER-Golgi v-SNAREs is an essential step for recruitment of Arf1p and coatomer, proteins that together form the COPI coat. ARF-GAP acts catalytically to recruit COPI components. Inclusion of v-SNAREs into COPI vesicles could be mediated by direct interaction with the coat. The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding.

Original languageEnglish
JournalJournal of Cell Biology
Volume157
Issue number3
Pages (from-to)395-404
Number of pages10
ISSN0021-9525
DOIs
Publication statusPublished - 29.04.2002

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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