Antibody-dependent cellular cytotoxicity in patients on chronic hemodialysis

Till Koch, Stefanie Derer, Matthias Staudinger, Kai Rossen, Pia Glorius, Matthias Peipp, Christian Kellner, Ulrich Kunzendorf, Thomas Valerius, Michael Dechant

    3 Citations (Scopus)

    Abstract

    Background: Antibody (Ab)-dependent cellular cytotoxicity (ADCC) is considered to be a relevant mechanism of action of Ab-based tumor therapies. However, knowledge about ADCC capacity of dialysis patients (DP) is limited. The aim of our study was to investigate if ADCC capacity of effector cells obtained from DP differed from those of healthy individuals (HI). Methods: First, we performed ADCC assays with isolated polymorphonuclear cells (PMN) and peripheral blood mononuclear cells (PBMC), mediated by the epidermal growth factor receptor Ab cetuximab or panitumumab. As cetuximab is of human IgG1 and panitumumab of human IgG2 isotype, both Abs differ in their affinity to Fcγ receptors and effector cell recruitment. Results: Using PMN as effectors, ADCC levels via panitumumab proved to be higher than via cetuximab, but did not differ between DP and HI. In contrast, IgG2-mediated ADCC with PBMC from DP was significantly enhanced compared to HI. IgG2 Abs predominantly bind to FcγRIIa. Within the PBMC, monocytes are the only cytotoxic cells physiologically expressing this receptor. ADCC experiments with isolated monocytes confirmed them to be the pivotal cells for the observed effect. Analysis of monocytes' Fc receptor expression demonstrated no difference between DP and HI, but monocytes of DP proved to be numerically increased and appeared preactivated. Conclusion: Our studies implicate that ADCC capacity is not impaired in DP and that it might particularly be reasonable to apply human IgG2 Abs as therapeutics for these patients.

    Original languageEnglish
    JournalAmerican Journal of Nephrology
    Volume38
    Issue number5
    Pages (from-to)379-387
    Number of pages9
    ISSN0250-8095
    DOIs
    Publication statusPublished - 11.2013

    Research Areas and Centers

    • Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)

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