An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Immanuel Andrä; Hanna Ulrich; Susi Dürr; Dominik Soll; Lynette Henkel; Corinne Angerpointner; Julia Ritter; Sabine Przibilla; Herbert Stadler; Manuel Effenberger; Dirk H. Busch; Matthias Schiemann

doi:10.1002/cyto.a.23964

An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Immanuel Andrä, Hanna Ulrich, Susi Dürr, Dominik Soll, Lynette Henkel, Corinne Angerpointner, Julia Ritter, Sabine Przibilla, Herbert Stadler, Manuel Effenberger, Dirk H. Busch, Matthias Schiemann^*

^*Corresponding author for this work

Institute of Systemic Inflammation Research

42 Citations (Scopus)

Abstract

Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.

Original language	English
Journal	Cytometry Part A
Volume	97
Issue number	2
Pages (from-to)	171-183
Number of pages	13
ISSN	1552-4922
DOIs	https://doi.org/10.1002/cyto.a.23964
Publication status	Published - 01.02.2020

Funding

This work was in part funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) ? ?Projektnummer 272983813? ? TRR 179. The authors thank Philipp Bruns (DKFZ, Heidelberg) and Lena Appel (MIH, M?nchen) for help with data processing and visualization of MicroArray data.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Areas and Centers

Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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title = "An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation",

abstract = "Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.",

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AU - Andrä, Immanuel

AU - Ulrich, Hanna

AU - Dürr, Susi

AU - Soll, Dominik

AU - Henkel, Lynette

AU - Angerpointner, Corinne

AU - Ritter, Julia

AU - Przibilla, Sabine

AU - Stadler, Herbert

AU - Effenberger, Manuel

AU - Busch, Dirk H.

AU - Schiemann, Matthias

PY - 2020/2/1

Y1 - 2020/2/1

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AB - Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments.

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An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation

Abstract

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