Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE

Maja Živković; Aleksandar Raković; Aleksandra Stanković

doi:10.1016/j.clinbiochem.2006.01.005

Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE

Maja Živković^*, Aleksandar Raković, Aleksandra Stanković

^*Corresponding author for this work

Department of Neurology

5 Citations (Scopus)

Abstract

Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.

Original language	English
Journal	Clinical Biochemistry
Volume	39
Issue number	6
Pages (from-to)	630-632
Number of pages	3
ISSN	0009-9120
DOIs	https://doi.org/10.1016/j.clinbiochem.2006.01.005
Publication status	Published - 01.06.2006

Funding

This work was supported by a Serbian Government Research Grant (5023). We greatly appreciate Dr. Dragan Alavantić for helpful suggestions during preparation of this paper.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 10 Reduced Inequalities

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10.1016/j.clinbiochem.2006.01.005

Cite this

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title = "Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE",

abstract = "Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.",

author = "Maja {\v Z}ivkovi{\'c} and Aleksandar Rakovi{\'c} and Aleksandra Stankovi{\'c}",

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AU - Živković, Maja

AU - Raković, Aleksandar

AU - Stanković, Aleksandra

PY - 2006/6/1

Y1 - 2006/6/1

N2 - Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.

AB - Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.

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U2 - 10.1016/j.clinbiochem.2006.01.005

DO - 10.1016/j.clinbiochem.2006.01.005

M3 - Journal articles

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JF - Clinical Biochemistry

IS - 6

ER -

Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE

Abstract

Funding

UN SDGs

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