TY - JOUR
T1 - Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE
AU - Živković, Maja
AU - Raković, Aleksandar
AU - Stanković, Aleksandra
PY - 2006/6/1
Y1 - 2006/6/1
N2 - Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.
AB - Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.
UR - http://www.scopus.com/inward/record.url?scp=33745477874&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2006.01.005
DO - 10.1016/j.clinbiochem.2006.01.005
M3 - Journal articles
C2 - 16458283
AN - SCOPUS:33745477874
SN - 0009-9120
VL - 39
SP - 630
EP - 632
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 6
ER -
