Projects per year
Cells isolated from pancreas have a remarkable potential for self-renewal and multilineage differentiation. We here present a comprehensive characterisation of stem/progenitor cells derived from exocrine parts of the adult rat pancreas. Using purified cells from either single colonies or even single-cell clones, we specifically demonstrate: (i) the cells contain the typical stem/progenitor cell markers alkaline phophatase, SSEA-1, Oct-4, CD9, Nestin, Pax6, CD44, α-Fetoprotein and Brachyury, demonstrated by immunocytochemistry and RT-PCR; (ii) the cells have the potential to differentiate into lineages of all three germ layers in vitro; (iii) a clonal analysis revealed that even cell lines derived from a single cell have stem/progenitor cell properties such as self-renewal and spontaneous differentiation into various cell lineages; (iv) the cells have the propensity to form three-dimensional, teratoma-like structures in vitro, which contain cells of different lineages; and (v) external stimuli can activate the generation of certain cell types. For instance, cells treated with retinoic acid show an increased expression of α-smooth muscle actin. These results suggest that exocrine glands, such as pancreas may be a potential source of adult stem/progenitor cells, suitable for cell therapy of degenerative diseases.
Research Areas and Centers
- Academic Focus: Center for Brain, Behavior and Metabolism (CBBM)
FingerprintDive into the research topics of 'Adult pancreatic stem/progenitor cells spontaneously differentiate in vitro into multiple cell lineages and form teratoma-like structures'. Together they form a unique fingerprint.
- 1 Finished
Schweikard, A., Anders, S., Barkhausen, J., Buzug, T., Erdmann, J., Fischer, B., Fischer, S., Habermann, J. K., Hartmann, E., Hilgenfeld, R., Hofmann, U., Klein, C., Kruse, C., Marshall, L., Martinetz, T., Mertins, A., Münte, T., Oltmanns, K., Schneider, S., Schunkert, H., Sczakiel, G., Tronnier, V. M., Vogel, A., Westermann, J. & Zillikens, D.
01.11.07 → 31.12.14
Project: DFG Funding