Adenylate cyclase 5 is required for melanophore and male pattern development in the guppy (Poecilia reticulata)

Verena A. Kottler*, Axel Künstner, Iris Koch, Matthias Flötenmeyer, Tobias Langenecker, Margarete Hoffmann, Eshita Sharma, Detlef Weigel, Christine Dreyer

*Corresponding author for this work
4 Citations (Scopus)

Abstract

Guppies (Poecilia reticulata) are colorful fish that have attracted the attention of pigmentation researchers for almost a century. Here, we report that the blond phenotype of the guppy is caused by a spontaneous mutation in the guppy ortholog of adenylate cyclase 5 (adcy5). Using double digest restriction site-associated DNA sequencing (ddRADseq) and quantitative trait locus (QTL) mapping, we linked the blond phenotype to a candidate region of 118 kb, in which we subsequently identified a 2-bp deletion in adcy5 that alters splicing and leads to a premature stop codon. We show that adcy5, which affects life span and melanoma growth in mouse, is required for melanophore development and formation of male orange pigmentation traits in the guppy. We find that some components of the male orange pattern are particularly sensitive to loss of Adcy5 function. Our work thus reveals a function for Adcy5 in patterning of fish color ornaments.

Original languageEnglish
JournalPigment Cell and Melanoma Research
Volume28
Issue number5
Pages (from-to)545-558
Number of pages14
ISSN1755-1471
DOIs
Publication statusPublished - 01.09.2015

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

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