TY - JOUR
T1 - Activation of cortical and inhibited differentiation of medullary epithelial cells in the thymus of lymphotoxin-beta receptor-deficient mice: An ultrastructural study
AU - Milićević, Novica M.
AU - Nohroudi, K.
AU - Milićević, Ž
AU - Westermann, J.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/2
Y1 - 2008/2
N2 - The reciprocal influences of thymic lymphocyte and nonlymphocyte populations, i.e. thymic cross-talk, are necessary for the proper maturation of thymocytes and the development/maintenance of thymic stromal microenvironments. Although the molecular influences exerted by thymic stromal cells on maturing thymocytes have been extensively studied, the identity of signalling molecules used by thymocytes to influence the thymic stromal cells is still largely unknown. Our study provides the first ultrastructural evidence that the functional lymphotoxin-beta receptor (LTβR) signalling pathway is engaged in the cross-talk between thymocytes and the thymic stromal cell population. We show that LTβR signalling is of the utmost significance for the preservation of the subcellular integrity of all thymic epithelial cells. In the absence of LTβR there is (1) hypertrophy and activation of cortical thymic epithelial cells, (2) the complete loss of fully differentiated medullary thymic epithelial cells, and (3) the inhibited differentiation of remaining medullary thymic epithelial cells with the appearance of prominent intercellular cysts in the thymic medulla.
AB - The reciprocal influences of thymic lymphocyte and nonlymphocyte populations, i.e. thymic cross-talk, are necessary for the proper maturation of thymocytes and the development/maintenance of thymic stromal microenvironments. Although the molecular influences exerted by thymic stromal cells on maturing thymocytes have been extensively studied, the identity of signalling molecules used by thymocytes to influence the thymic stromal cells is still largely unknown. Our study provides the first ultrastructural evidence that the functional lymphotoxin-beta receptor (LTβR) signalling pathway is engaged in the cross-talk between thymocytes and the thymic stromal cell population. We show that LTβR signalling is of the utmost significance for the preservation of the subcellular integrity of all thymic epithelial cells. In the absence of LTβR there is (1) hypertrophy and activation of cortical thymic epithelial cells, (2) the complete loss of fully differentiated medullary thymic epithelial cells, and (3) the inhibited differentiation of remaining medullary thymic epithelial cells with the appearance of prominent intercellular cysts in the thymic medulla.
UR - http://www.scopus.com/inward/record.url?scp=38849088601&partnerID=8YFLogxK
U2 - 10.1111/j.1469-7580.2007.00851.x
DO - 10.1111/j.1469-7580.2007.00851.x
M3 - Journal articles
C2 - 18194204
AN - SCOPUS:38849088601
SN - 0021-8782
VL - 212
SP - 114
EP - 124
JO - Journal of Anatomy
JF - Journal of Anatomy
IS - 2
ER -