TY - JOUR
T1 - Absence of Both Thyroid Hormone Transporters MCT8 and OATP1C1 Impairs Neural Stem Cell Fate in the Adult Mouse Subventricular Zone
AU - Luongo, Cristina
AU - Butruille, Lucile
AU - Sébillot, Anthony
AU - Le Blay, Karine
AU - Schwaninger, Markus
AU - Heuer, Heike
AU - Demeneix, Barbara A.
AU - Remaud, Sylvie
N1 - Funding Information:
The authors would like to thank S. Sosinsky and F. Uridat for the excellent animal care. We also thank the ImagoSeine platform of the Institute Jacques Monod, Université René Descartes for the imaging advice. This work was supported by the CNRS and the EU H2020 contract Thyrage (grant n° 666869 ). H.H. and M.S. are supported by the Deutsche Forschungsgemeinschaft (DFG; TR 296/1-P01 ).
Publisher Copyright:
© 2020 The Authors
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/2/9
Y1 - 2021/2/9
N2 - Adult neural stem cell (NSC) generation in vertebrate brains requires thyroid hormones (THs). How THs enter the NSC population is unknown, although TH availability determines proliferation and neuronal versus glial progenitor determination in murine subventricular zone (SVZ) NSCs. Mice display neurological signs of the severely disabling human disease, Allan-Herndon-Dudley syndrome, if they lack both MCT8 and OATP1C1 transporters, or MCT8 and deiodinase type 2. We analyzed the distribution of MCT8 and OATP1C1 in adult mouse SVZ. Both are strongly expressed in NSCs and at a lower level in neuronal cell precursors but not in oligodendrocyte progenitors. Next, we analyzed Mct8/Oatp1c1 double-knockout mice, where brain uptake of THs is strongly reduced. NSC proliferation and determination to neuronal fates were severely affected, but not SVZ-oligodendroglial progenitor generation. This work highlights how tight control of TH availability determines NSC function and glial-neuron cell-fate choice in adult brains.
AB - Adult neural stem cell (NSC) generation in vertebrate brains requires thyroid hormones (THs). How THs enter the NSC population is unknown, although TH availability determines proliferation and neuronal versus glial progenitor determination in murine subventricular zone (SVZ) NSCs. Mice display neurological signs of the severely disabling human disease, Allan-Herndon-Dudley syndrome, if they lack both MCT8 and OATP1C1 transporters, or MCT8 and deiodinase type 2. We analyzed the distribution of MCT8 and OATP1C1 in adult mouse SVZ. Both are strongly expressed in NSCs and at a lower level in neuronal cell precursors but not in oligodendrocyte progenitors. Next, we analyzed Mct8/Oatp1c1 double-knockout mice, where brain uptake of THs is strongly reduced. NSC proliferation and determination to neuronal fates were severely affected, but not SVZ-oligodendroglial progenitor generation. This work highlights how tight control of TH availability determines NSC function and glial-neuron cell-fate choice in adult brains.
UR - http://www.scopus.com/inward/record.url?scp=85100016962&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/caf59829-c0c4-3f3a-b270-7b3157bd7d0f/
U2 - 10.1016/j.stemcr.2020.12.009
DO - 10.1016/j.stemcr.2020.12.009
M3 - Journal articles
C2 - 33450189
AN - SCOPUS:85100016962
SN - 2213-6711
VL - 16
SP - 337
EP - 353
JO - Stem Cell Reports
JF - Stem Cell Reports
IS - 2
ER -
