A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy

Sebastian Karpf, Matthias Eibl, Wolfgang Wieser, Thomas Klein, Robert Huber*

*Corresponding author for this work
34 Citations (Scopus)


Raman sensing and microscopy are among the most specific optical technologies to identify the chemical compounds of unknown samples, and to enable label-free biomedical imaging. Here we present a method for stimulated Raman scattering spectroscopy and imaging with a time-encoded (TICO) Raman concept. We use continuous wave, rapidly wavelength-swept probe lasers and combine them with a short-duty-cycle actively modulated pump laser. Hence, we achieve high stimulated Raman gain signal levels, while still benefitting from the narrow linewidth and low noise of continuous wave operation. Our all-fibre TICO-Raman setup uses a Fourier domain mode-locked laser source to achieve a unique combination of high speed, broad spectral coverage (750-3,150 cm -1) and high resolution (0.5 cm -1). The Raman information is directly encoded and acquired in time. We demonstrate quantitative chemical analysis of a solvent mixture and hyperspectral Raman microscopy with molecular contrast of plant cells.

Original languageEnglish
Article number7784
JournalNature Communications
Publication statusPublished - 17.04.2015


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