A THz transparent 3D printed microfluidic cell for small angle x-ray scattering

S. Schewa; M. A. Schroer; T. Zickmantel; Y. H. Song; C. E. Blanchet; A. Yu Gruzinov; G. Katona; D. I. Svergun; M. Roessle

doi:10.1063/5.0004706

A THz transparent 3D printed microfluidic cell for small angle x-ray scattering

S. Schewa, M. A. Schroer, T. Zickmantel, Y. H. Song, C. E. Blanchet, A. Yu Gruzinov, G. Katona, D. I. Svergun, M. Roessle^*

^*Corresponding author for this work

Institute of Physics

5 Citations (Scopus)

Abstract

Excitation frequencies in the terahertz (THz) range are expected to lead to functionally relevant domain movements within the biological macromolecules such as proteins. The possibility of examining such movements in an aqueous environment is particularly valuable since here proteins are not deprived of any motional degrees of freedom. Small angle x-ray scattering (SAXS) is a powerful method to study the structure and domain movements of proteins in solution. Here, we present a microfluidic cell for SAXS experiments, which is also transparent for THz radiation. Specifically, cell dimensions and material were optimized for both radiation sources. In addition, the polystyrene cell can be 3D printed and easily assembled. We demonstrate the practicality of our design for SAXS measurements on several proteins in solution.

Original language	English
Article number	084101
Journal	Review of Scientific Instruments
Volume	91
Issue number	8
ISSN	0034-6748
DOIs	https://doi.org/10.1063/5.0004706
Publication status	Published - 01.08.2020

Funding

All authors thank the German Bundesministerium für Bil-dung und Forschung/Röntgen-Ångström cluster project “TT-SAS” (Grant No. 05K16YEA).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

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10.1063/5.0004706

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@article{23d187d021b445fea154ccfc38c51ac9,

title = "A THz transparent 3D printed microfluidic cell for small angle x-ray scattering",

abstract = "Excitation frequencies in the terahertz (THz) range are expected to lead to functionally relevant domain movements within the biological macromolecules such as proteins. The possibility of examining such movements in an aqueous environment is particularly valuable since here proteins are not deprived of any motional degrees of freedom. Small angle x-ray scattering (SAXS) is a powerful method to study the structure and domain movements of proteins in solution. Here, we present a microfluidic cell for SAXS experiments, which is also transparent for THz radiation. Specifically, cell dimensions and material were optimized for both radiation sources. In addition, the polystyrene cell can be 3D printed and easily assembled. We demonstrate the practicality of our design for SAXS measurements on several proteins in solution. ",

author = "S. Schewa and Schroer, \{M. A.\} and T. Zickmantel and Song, \{Y. H.\} and Blanchet, \{C. E.\} and Gruzinov, \{A. Yu\} and G. Katona and Svergun, \{D. I.\} and M. Roessle",

note = "Funding Information: All authors thank the German Bundesministerium f{\"u}r Bil-dung und Forschung/R{\"o}ntgen-{\AA}ngstr{\"o}m cluster project “TT-SAS” (Grant No. 05K16YEA). Publisher Copyright: {\textcopyright} 2020 Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

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doi = "10.1063/5.0004706",

language = "English",

volume = "91",

journal = "Review of Scientific Instruments",

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AU - Schewa, S.

AU - Schroer, M. A.

AU - Zickmantel, T.

AU - Song, Y. H.

AU - Blanchet, C. E.

AU - Gruzinov, A. Yu

AU - Katona, G.

AU - Svergun, D. I.

AU - Roessle, M.

N1 - Funding Information: All authors thank the German Bundesministerium für Bil-dung und Forschung/Röntgen-Ångström cluster project “TT-SAS” (Grant No. 05K16YEA). Publisher Copyright: © 2020 Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - Excitation frequencies in the terahertz (THz) range are expected to lead to functionally relevant domain movements within the biological macromolecules such as proteins. The possibility of examining such movements in an aqueous environment is particularly valuable since here proteins are not deprived of any motional degrees of freedom. Small angle x-ray scattering (SAXS) is a powerful method to study the structure and domain movements of proteins in solution. Here, we present a microfluidic cell for SAXS experiments, which is also transparent for THz radiation. Specifically, cell dimensions and material were optimized for both radiation sources. In addition, the polystyrene cell can be 3D printed and easily assembled. We demonstrate the practicality of our design for SAXS measurements on several proteins in solution.

AB - Excitation frequencies in the terahertz (THz) range are expected to lead to functionally relevant domain movements within the biological macromolecules such as proteins. The possibility of examining such movements in an aqueous environment is particularly valuable since here proteins are not deprived of any motional degrees of freedom. Small angle x-ray scattering (SAXS) is a powerful method to study the structure and domain movements of proteins in solution. Here, we present a microfluidic cell for SAXS experiments, which is also transparent for THz radiation. Specifically, cell dimensions and material were optimized for both radiation sources. In addition, the polystyrene cell can be 3D printed and easily assembled. We demonstrate the practicality of our design for SAXS measurements on several proteins in solution.

UR - https://www.scopus.com/pages/publications/85089392799

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JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

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M1 - 084101

ER -

A THz transparent 3D printed microfluidic cell for small angle x-ray scattering

Abstract

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UN SDGs

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