A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid

S Goletz; C Probst; L Komorowski; W Schlumberger; K Fechner; N van Beek; M M Holtsche; A Recke; K B Yancey; T Hashimoto; F Antonicelli; G Di Zenzo; D Zillikens; W Stöcker; E Schmidt

doi:10.1111/bjd.17202

A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid

S Goletz, C Probst, L Komorowski, W Schlumberger, K Fechner, N van Beek, M M Holtsche, A Recke, K B Yancey, T Hashimoto, F Antonicelli, G Di Zenzo, D Zillikens, W Stöcker, E Schmidt

Abstract

BACKGROUND: Antilaminin 332 mucous membrane pemphigoid (MMP) is an autoimmune subepidermal blistering disease with predominant mucosal involvement and autoantibodies against laminin 332. Malignancies have been associated with this disease; however, no standardized detection system for antilaminin 332 serum antibodies is widely available.

OBJECTIVES: Development of a sensitive and specific assay for the detection of antilaminin 332 antibodies.

METHODS: An indirect immunofluorescence (IF) assay using recombinant laminin 332 was developed and probed with a large number of antilaminin 332 MMP patient sera (n = 93), as well as sera from patients with antilaminin 332-negative MMP (n = 153), bullous pemphigoid (n = 20), pemphigus vulgaris (n = 20) and noninflammatory dermatoses (n = 22), and healthy blood donors (n = 100).

RESULTS: In the novel IF assay, sensitivities with the laminin 332 heterotrimer and the individual α3, β3 and γ2 chains were 77%, 43%, 41% and 13%, respectively, with specificities of 100% for each substrate. The sensitivity for the heterotrimer increased when an anti-IgG4 enriched antitotal IgG conjugate was applied. Antilaminin 332 reactivity paralleled disease activity and was associated with malignancies in 25% of patients with antilaminin 332 MMP.

CONCLUSIONS: The novel IF-based assay will facilitate the serological diagnosis of antilaminin 332 MMP and may help to identify patients at risk of a malignancy.

Original language	English
Journal	British Journal of Dermatology
Volume	180
Issue number	1
Pages (from-to)	149-156
Number of pages	8
ISSN	0007-0963
DOIs	https://doi.org/10.1111/bjd.17202
Publication status	Published - 01.01.2019

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10.1111/bjd.17202

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Goletz, S., Probst, C., Komorowski, L., Schlumberger, W., Fechner, K., van Beek, N., Holtsche, M. M., Recke, A., Yancey, K. B., Hashimoto, T., Antonicelli, F., Di Zenzo, G., Zillikens, D., Stöcker, W., & Schmidt, E. (2019). A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid. British Journal of Dermatology, 180(1), 149-156. https://doi.org/10.1111/bjd.17202

@article{2ea1d42fa01143eeb91f74b5119b53d3,

title = "A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid",

abstract = "BACKGROUND: Antilaminin 332 mucous membrane pemphigoid (MMP) is an autoimmune subepidermal blistering disease with predominant mucosal involvement and autoantibodies against laminin 332. Malignancies have been associated with this disease; however, no standardized detection system for antilaminin 332 serum antibodies is widely available.OBJECTIVES: Development of a sensitive and specific assay for the detection of antilaminin 332 antibodies.METHODS: An indirect immunofluorescence (IF) assay using recombinant laminin 332 was developed and probed with a large number of antilaminin 332 MMP patient sera (n = 93), as well as sera from patients with antilaminin 332-negative MMP (n = 153), bullous pemphigoid (n = 20), pemphigus vulgaris (n = 20) and noninflammatory dermatoses (n = 22), and healthy blood donors (n = 100).RESULTS: In the novel IF assay, sensitivities with the laminin 332 heterotrimer and the individual α3, β3 and γ2 chains were 77%, 43%, 41% and 13%, respectively, with specificities of 100% for each substrate. The sensitivity for the heterotrimer increased when an anti-IgG4 enriched antitotal IgG conjugate was applied. Antilaminin 332 reactivity paralleled disease activity and was associated with malignancies in 25% of patients with antilaminin 332 MMP.CONCLUSIONS: The novel IF-based assay will facilitate the serological diagnosis of antilaminin 332 MMP and may help to identify patients at risk of a malignancy.",

author = "S Goletz and C Probst and L Komorowski and W Schlumberger and K Fechner and {van Beek}, N and Holtsche, {M M} and A Recke and Yancey, {K B} and T Hashimoto and F Antonicelli and {Di Zenzo}, G and D Zillikens and W St{\"o}cker and E Schmidt",

note = "{\textcopyright} 2018 British Association of Dermatologists.",

year = "2019",

month = jan,

day = "1",

doi = "10.1111/bjd.17202",

language = "English",

volume = "180",

pages = "149--156",

journal = "British Journal of Dermatology",

issn = "0007-0963",

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Goletz, S, Probst, C, Komorowski, L, Schlumberger, W, Fechner, K, van Beek, N , Holtsche, MM , Recke, A, Yancey, KB, Hashimoto, T, Antonicelli, F, Di Zenzo, G, Zillikens, D, Stöcker, W & Schmidt, E 2019, 'A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid', British Journal of Dermatology, vol. 180, no. 1, pp. 149-156. https://doi.org/10.1111/bjd.17202

TY - JOUR

T1 - A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid

AU - Goletz, S

AU - Probst, C

AU - Komorowski, L

AU - Schlumberger, W

AU - Fechner, K

AU - van Beek, N

AU - Holtsche, M M

AU - Recke, A

AU - Yancey, K B

AU - Hashimoto, T

AU - Antonicelli, F

AU - Di Zenzo, G

AU - Zillikens, D

AU - Stöcker, W

AU - Schmidt, E

PY - 2019/1/1

Y1 - 2019/1/1

N2 - BACKGROUND: Antilaminin 332 mucous membrane pemphigoid (MMP) is an autoimmune subepidermal blistering disease with predominant mucosal involvement and autoantibodies against laminin 332. Malignancies have been associated with this disease; however, no standardized detection system for antilaminin 332 serum antibodies is widely available.OBJECTIVES: Development of a sensitive and specific assay for the detection of antilaminin 332 antibodies.METHODS: An indirect immunofluorescence (IF) assay using recombinant laminin 332 was developed and probed with a large number of antilaminin 332 MMP patient sera (n = 93), as well as sera from patients with antilaminin 332-negative MMP (n = 153), bullous pemphigoid (n = 20), pemphigus vulgaris (n = 20) and noninflammatory dermatoses (n = 22), and healthy blood donors (n = 100).RESULTS: In the novel IF assay, sensitivities with the laminin 332 heterotrimer and the individual α3, β3 and γ2 chains were 77%, 43%, 41% and 13%, respectively, with specificities of 100% for each substrate. The sensitivity for the heterotrimer increased when an anti-IgG4 enriched antitotal IgG conjugate was applied. Antilaminin 332 reactivity paralleled disease activity and was associated with malignancies in 25% of patients with antilaminin 332 MMP.CONCLUSIONS: The novel IF-based assay will facilitate the serological diagnosis of antilaminin 332 MMP and may help to identify patients at risk of a malignancy.

AB - BACKGROUND: Antilaminin 332 mucous membrane pemphigoid (MMP) is an autoimmune subepidermal blistering disease with predominant mucosal involvement and autoantibodies against laminin 332. Malignancies have been associated with this disease; however, no standardized detection system for antilaminin 332 serum antibodies is widely available.OBJECTIVES: Development of a sensitive and specific assay for the detection of antilaminin 332 antibodies.METHODS: An indirect immunofluorescence (IF) assay using recombinant laminin 332 was developed and probed with a large number of antilaminin 332 MMP patient sera (n = 93), as well as sera from patients with antilaminin 332-negative MMP (n = 153), bullous pemphigoid (n = 20), pemphigus vulgaris (n = 20) and noninflammatory dermatoses (n = 22), and healthy blood donors (n = 100).RESULTS: In the novel IF assay, sensitivities with the laminin 332 heterotrimer and the individual α3, β3 and γ2 chains were 77%, 43%, 41% and 13%, respectively, with specificities of 100% for each substrate. The sensitivity for the heterotrimer increased when an anti-IgG4 enriched antitotal IgG conjugate was applied. Antilaminin 332 reactivity paralleled disease activity and was associated with malignancies in 25% of patients with antilaminin 332 MMP.CONCLUSIONS: The novel IF-based assay will facilitate the serological diagnosis of antilaminin 332 MMP and may help to identify patients at risk of a malignancy.

UR - http://www.mendeley.com/research/sensitive-specific-assay-serological-diagnosis-antilaminin-332-mucous-membrane-pemphigoid

U2 - 10.1111/bjd.17202

DO - 10.1111/bjd.17202

M3 - Journal articles

C2 - 30216412

SN - 0007-0963

VL - 180

SP - 149

EP - 156

JO - British Journal of Dermatology

JF - British Journal of Dermatology

IS - 1

ER -

A sensitive and specific assay for the serological diagnosis of antilaminin 332 mucous membrane pemphigoid

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