Objective: To present a live birth after freezing and thawing of biopsied oocytes. Design: Case report. Setting: Artificial reproduction unit of a university hospital. Patient(s): A primary infertile couple with asthenoteratozoospermia and repeated failures of intracytoplasmic sperm injection (ICSI). Intervention(s): Screening of aneuploidy during the fourth ICSI cycle with polar body biopsy (PB) for repeated failures of artificial reproductive techniques and a transfer of a cryopreserved day 2 embryo derived from cryopreserved zygotes with slow-rate freezing after PB. Main Outcomes Measure(s): Live birth, viability, and survival. Result(s): A successful pregnancy and a live birth were presented after a transfer of day 2 embryos derived from oocytes that underwent PB and subsequent cryopreservation. Conclusion(s): Pregnancy can be obtained subsequent to cryopreservation and thawing after PB.