@article{43b864c7a918411a80ff951f5b3218f9,
title = "A framework for tracer-based metabolism in mammalian cells by NMR",
abstract = "Metabolism changes extensively during the normal proliferation and differentiation of mammalian cells, and in cancer and inflammatory diseases. Since changes in the metabolic network reflect interactions between genetic, epigenetic and environmental changes, it is helpful to study the flow of label from isotopically labelled precursors into other metabolites rather than static metabolite levels. For this Nuclear Magnetic Resonance (NMR) spectroscopy is an attractive technique as it can quantify site-specific label incorporation. However, for applications using human cells and cell lines, the challenge is to optimize the process to maximize sensitivity and reproducibility. Here we present a new framework to analyze metabolism in mammalian cell lines and primary cells, covering the workflow from the preparation of cells to the acquisition and analysis of NMR spectra. We have applied this new approach in hematological and liver cancer cell lines and confirm the feasibility of tracer-based metabolism in primary liver cells.",
author = "Raquel Saborano and Zuhal Eraslan and Jennie Roberts and Khanim, {Farhat L.} and Lalor, {Patricia F.} and Reed, {Michelle A.C.} and G{\"u}nther, {Ulrich L.}",
note = "Funding Information: Much of the work presented is based on the FP7 Metaflux project (FP7-PEOPLE-ITN-2010–264780). We thank the EC for supporting ZE and RS within the EUROPOL-ITN project (H2020-MSCA-ITN-2014-642773), PhenoMeNal for supporting software development (H2020-EINFRA-2014-2-654241), and BBSRC for supporting JR in the context of a BBSRC CASE studentship. We are also grateful to the Wellcome Trust for supporting access to NMR instruments at the Henry Wellcome Building for Biomolecular NMR in Birmingham (grant number 208400/Z/17/Z). We are also grateful for access to NMR instrumentation at the BMRZ NMR centre in Frankfurt with support from INext, and thank Christian Richter for technical assistance. This report includes independent research funded by the NIHR Birmingham Biomedical Research Centre at the University Hospitals Birmingham NHS Foundation Trust and the University of Birmingham. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health. Publisher Copyright: {\textcopyright} 2019, The Author(s). Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-018-37525-3",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
number = "1",
}
