Abstract
Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.
| Original language | English |
|---|---|
| Journal | Nature Methods |
| Volume | 8 |
| Issue number | 4 |
| Pages (from-to) | 315-317 |
| Number of pages | 3 |
| ISSN | 1548-7091 |
| DOIs | |
| Publication status | Published - 01.04.2011 |
| Externally published | Yes |
Funding
F.-J.M. is supported by an Else-Kröner Fresenius Stiftung fellowship. J.F.L. is supported by grants from the California Institute for Regenerative Medicine (RT1-01108, TR1-01250 and CL1-00502), the US National Institutes of Health (R21 MH087925), the Bill and Melinda Gates Foundation, the Esther O’Keeffe Foundation and the Millipore Foundation. B.M.S. is supported by Bayer Technology Services GmbH and the Deutsche Forschungsgemeinschaft (GSC 111). L.C.L. is supported by a US National Institutes of Health National Institute of Child Health and Human Development K12 Career Development award. E.P.P. was supported by New York State Stem Cell Science grant N08T-060. We thank C. Lynch and H. Tran for preparing the samples and running the arrays; A. Schuppert, S. Peterson and K. Nazor for comments, criticisms and reading the manuscript for clarity; M. Sadelain (Memorial Sloan-Kettering Cancer Center) for providing samples and data; K. Haden and I. Mikoulitch (Illumina) for help with handling Illumina BeadArray data formats and letting us use the idat.reader.dll program module in PluriTest RIA; and C. Becker, D. Barker and A. Fritz for helpful discussions.