4D microscopic optical coherence tomography imaging of ex vivo mucus transport

M. Münter; H. Schulz-Hildebrandt; Mario Pieper; P. König; G. Hüttmann

doi:10.1117/12.2527138

4D microscopic optical coherence tomography imaging of ex vivo mucus transport

M. Münter^*, H. Schulz-Hildebrandt, Mario Pieper, P. König, G. Hüttmann

^*Corresponding author for this work

Abstract

Optical coherence tomography (OCT) with microscopic resolution is suited for the investigation of dynamic processes on cellular level. Most existing setups for microscopic OCT (mOCT) are able to acquire A-Scans at 100 kHz, hence they are suitable for displaying only B-Scans in real-time. We present an mOCT setup with a new high-speed spectrometer, which is capable of acquiring up to 600.000 A-scans/s. Customized software allows to image mOCT volumes with micrometer resolution at video rate. Therefore, we are able to visualize functional processes on a cellular level in three dimensions over time. Here, we present 2D and 4D data of ex vivo human lung. Recording tens of high-resolution OCT volumes per seconds becomes possible faster than previous setup for OCT with microscopic resolution.

Original language	English
Title of host publication	Optical Coherence Imaging Techniques and Imaging in Scattering Media III
Editors	Maciej Wojtkowski, Stephen A. Boppart, Wang-Yuhl Oh
Number of pages	53
Volume	11078
Publisher	SPIE
Publication date	19.07.2019
ISBN (Print)	978-155752820-9
DOIs	https://doi.org/10.1117/12.2527138
Publication status	Published - 19.07.2019
Event	European Conference on Biomedical Optics - Munich, Germany Duration: 23.06.2019 → 25.06.2019 Conference number: 142118

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 9 Industry, Innovation, and Infrastructure

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Academic Focus: Biomedical Engineering

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10.1117/12.2527138

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Münter, M., Schulz-Hildebrandt, H., Pieper, M., König, P., & Hüttmann, G. (2019). 4D microscopic optical coherence tomography imaging of ex vivo mucus transport. In M. Wojtkowski, S. A. Boppart, & W.-Y. Oh (Eds.), Optical Coherence Imaging Techniques and Imaging in Scattering Media III (Vol. 11078). (Optics InfoBase Conference Papers; Vol. 11078). SPIE. https://doi.org/10.1117/12.2527138

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title = "4D microscopic optical coherence tomography imaging of ex vivo mucus transport",

abstract = "Optical coherence tomography (OCT) with microscopic resolution is suited for the investigation of dynamic processes on cellular level. Most existing setups for microscopic OCT (mOCT) are able to acquire A-Scans at 100 kHz, hence they are suitable for displaying only B-Scans in real-time. We present an mOCT setup with a new high-speed spectrometer, which is capable of acquiring up to 600.000 A-scans/s. Customized software allows to image mOCT volumes with micrometer resolution at video rate. Therefore, we are able to visualize functional processes on a cellular level in three dimensions over time. Here, we present 2D and 4D data of ex vivo human lung. Recording tens of high-resolution OCT volumes per seconds becomes possible faster than previous setup for OCT with microscopic resolution.",

author = "M. M{\"u}nter and H. Schulz-Hildebrandt and Mario Pieper and P. K{\"o}nig and G. H{\"u}ttmann",

year = "2019",

month = jul,

day = "19",

doi = "10.1117/12.2527138",

language = "English",

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volume = "11078",

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editor = "Wojtkowski, \{Maciej \} and Boppart, \{Stephen A. \} and Oh, \{Wang-Yuhl \}",

booktitle = "Optical Coherence Imaging Techniques and Imaging in Scattering Media III",

note = "European Conference on Biomedical Optics , ECBO\_2019 ; Conference date: 23-06-2019 Through 25-06-2019",

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Münter, M, Schulz-Hildebrandt, H, Pieper, M , König, P & Hüttmann, G 2019, 4D microscopic optical coherence tomography imaging of ex vivo mucus transport. in M Wojtkowski, SA Boppart & W-Y Oh (eds), Optical Coherence Imaging Techniques and Imaging in Scattering Media III. vol. 11078, Optics InfoBase Conference Papers, vol. 11078, SPIE, European Conference on Biomedical Optics , Munich, Bavaria, Germany, 23.06.19. https://doi.org/10.1117/12.2527138

4D microscopic optical coherence tomography imaging of ex vivo mucus transport. / Münter, M.; Schulz-Hildebrandt, H.; Pieper, Mario et al.
Optical Coherence Imaging Techniques and Imaging in Scattering Media III. ed. / Maciej Wojtkowski; Stephen A. Boppart; Wang-Yuhl Oh. Vol. 11078 SPIE, 2019. (Optics InfoBase Conference Papers; Vol. 11078).

Research output: Chapters in Books/Reports/Conference Proceedings › Conference contribution › peer-review

TY - GEN

T1 - 4D microscopic optical coherence tomography imaging of ex vivo mucus transport

AU - Münter, M.

AU - Schulz-Hildebrandt, H.

AU - Pieper, Mario

AU - König, P.

AU - Hüttmann, G.

N1 - Conference code: 142118

PY - 2019/7/19

Y1 - 2019/7/19

N2 - Optical coherence tomography (OCT) with microscopic resolution is suited for the investigation of dynamic processes on cellular level. Most existing setups for microscopic OCT (mOCT) are able to acquire A-Scans at 100 kHz, hence they are suitable for displaying only B-Scans in real-time. We present an mOCT setup with a new high-speed spectrometer, which is capable of acquiring up to 600.000 A-scans/s. Customized software allows to image mOCT volumes with micrometer resolution at video rate. Therefore, we are able to visualize functional processes on a cellular level in three dimensions over time. Here, we present 2D and 4D data of ex vivo human lung. Recording tens of high-resolution OCT volumes per seconds becomes possible faster than previous setup for OCT with microscopic resolution.

AB - Optical coherence tomography (OCT) with microscopic resolution is suited for the investigation of dynamic processes on cellular level. Most existing setups for microscopic OCT (mOCT) are able to acquire A-Scans at 100 kHz, hence they are suitable for displaying only B-Scans in real-time. We present an mOCT setup with a new high-speed spectrometer, which is capable of acquiring up to 600.000 A-scans/s. Customized software allows to image mOCT volumes with micrometer resolution at video rate. Therefore, we are able to visualize functional processes on a cellular level in three dimensions over time. Here, we present 2D and 4D data of ex vivo human lung. Recording tens of high-resolution OCT volumes per seconds becomes possible faster than previous setup for OCT with microscopic resolution.

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U2 - 10.1117/12.2527138

DO - 10.1117/12.2527138

M3 - Conference contribution

SN - 978-155752820-9

VL - 11078

T3 - Optics InfoBase Conference Papers

BT - Optical Coherence Imaging Techniques and Imaging in Scattering Media III

A2 - Wojtkowski, Maciej

A2 - Boppart, Stephen A.

A2 - Oh, Wang-Yuhl

PB - SPIE

T2 - European Conference on Biomedical Optics

Y2 - 23 June 2019 through 25 June 2019

ER -

4D microscopic optical coherence tomography imaging of ex vivo mucus transport

Abstract

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