SPP 1468, Subproject: Pasteurella multocida toxin as a tool to modulate bone and immune cell differentiation

  • Hieke-Kubatzky, Katharina (Principal Investigator (PI))
  • Dörner, Thomas (Associated Staff)
  • Garbe, Annette (Associated Staff)
  • Manz, Rudolf (Associated Staff)

Project: DFG ProjectsDFG Joint Research: Priority Programs

Project Details

Description

The project will investigate the connection between B-cell and osteoclast differentiation, using the bacterial toxin from Pasteurella multocida (PMT) as a model system. PMT is a potent mitogen with known osteoclastic properties that stimulates differentiation of bone marrow cells into osteoclasts and inhibits osteoblast function, respectively. The aim of the project is to further elucidate the mechanism of PMTinduced osteoclast differentiation and to define the role of B-cells in that process. We aim to characterise the PMT-generated B-cell and osteoclast populations and their interaction or dependence of each other. We will investigate the mechanism of that interaction on a molecular level by characterising the secreted factors and by comparing the secretome and proteome of PMT and IL7, M-CSF/RANKL or LPS derived osteoclasts. In addition, we aim to define the signalling networks involved in the interplay between B-cells and osteoclasts, with a focus on anti-apoptotic signalling through Akt and Pim kinases and the importance of migration and adhesion to further expand our understanding of the regulation of bone destruction by immunological processes.
Statusfinished
Effective start/end date01.01.1031.12.17

Collaborative partners

  • Ruprecht-Karls Universität Heidelberg (Joint applicant, Co-PI) (lead)
  • Technische Universität Dresden (Associated Staff)
  • Charite - Universitatsmedizin Berlin (Associated Staff)

Research Areas and Centers

  • Academic Focus: Center for Infection and Inflammation Research (ZIEL)

DFG Research Classification Scheme

  • 205-18 Rheumatology
  • 204-05 Immunology

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