Ultrafast Spectral Tuning of a Fiber Laser for Time-Encoded Multiplex Coherent Raman Scattering Microscopy

Thomas Gottschall, Tobias Meyer-Zedler, Matthias Eibl, Tom Pfeiffer, Hubertus Hakert, Michael Schmitt, Robert Huber, Andreas Tünnermann, Jens Limpert, Juergen Popp

Abstract

Coherent Raman scattering microscopy utilizing bioorthogonal tagging approaches like isotope or alkyne labeling allows for a targeted monitoring of spatial distribution and dynamics of small molecules of interest in cells, tissues, and other complex biological matrices. To fully exploit this approach in terms of real-time monitoring of several Raman tags, e.g., to study drug uptake dynamics, extremely fast tunable lasers are needed. Here, we present a laser concept without moving parts and fully electronically controlled for the quasi-simultaneous acquisition of coherent anti-Stokes Raman scattering images at multiple Raman resonances. The laser concept is based on the combination of a low noise and spectrally narrow Fourier domain mode-locked laser seeding a compact four wave mixing-based high-power fiber-based optical parametric amplifier.

OriginalspracheEnglisch
ZeitschriftThe Journal of Physical Chemistry B
Jahrgang127
Ausgabenummer11
Seiten (von - bis)2375-2380
Seitenumfang6
DOIs
PublikationsstatusVeröffentlicht - 23.03.2023

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