Transcription factor AP2alpha (TFAP2a) regulates differentiation and proliferation of neuroblastoma cells

Johannes H. Schulte; Jutta Kirfel; Soyoung Lim; Alexander Schramm; Nicolaus Friedrichs; Hedwig E. Deubzer; Olaf Witt; Angelika Eggert; Reinhard Buettner

doi:10.1016/j.canlet.2008.05.039

Transcription factor AP2alpha (TFAP2a) regulates differentiation and proliferation of neuroblastoma cells

Johannes H. Schulte^*, Jutta Kirfel, Soyoung Lim, Alexander Schramm, Nicolaus Friedrichs, Hedwig E. Deubzer, Olaf Witt, Angelika Eggert, Reinhard Buettner

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Pathologie

27 Zitate (Scopus)

Abstract

Neuroblastoma, the most common extracranial solid tumour of childhood, is derived from neural crest progenitor cells. The TFAP2a transcription factor regulates neural crest patterning. We analysed TFAP2a protein expression in 97 primary neuroblastic tumors and report that TFAP2a was strongly expressed in poorly differentiated neuroblastomas. TFAP2a expression in tumor cells of differentiated neuroblastic tumors was below detection. TFAP2a was strongly expressed in 4 of 6 neuroblastoma cell lines tested, and TFAP2a siRNA mediated knock down in SH-EP cells reduced proliferation and induced a more differentiated phenotype associated with an increase in the expression of the differentiation marker neurotensin.

Originalsprache	Englisch
Zeitschrift	Cancer Letters
Jahrgang	271
Ausgabenummer	1
Seiten (von - bis)	56-63
Seitenumfang	8
ISSN	0304-3835
DOIs	https://doi.org/10.1016/j.canlet.2008.05.039
Publikationsstatus	Veröffentlicht - 18.11.2008

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We thank Francoise Halley, Theresia Walter and Birte Sönnichsen (Cenix BioScience GmbH, Dresden, Germany) for performing the siRNA experiments; Sabine Dreesmann for excellent technical assistance; Kathy Astrahantseff for critical reading of the manuscript as well as Barbara Hero and the German Neuroblastoma Study Group for providing clinical data. A.E., O.W. and A.S. were supported by grants from the National Genome Research Network (NGFN), R.B. was supported by a grant from the DFG.

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10.1016/j.canlet.2008.05.039

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@article{ec3377faec3240daaa16ec553dc26a19,

title = "Transcription factor AP2alpha (TFAP2a) regulates differentiation and proliferation of neuroblastoma cells",

abstract = "Neuroblastoma, the most common extracranial solid tumour of childhood, is derived from neural crest progenitor cells. The TFAP2a transcription factor regulates neural crest patterning. We analysed TFAP2a protein expression in 97 primary neuroblastic tumors and report that TFAP2a was strongly expressed in poorly differentiated neuroblastomas. TFAP2a expression in tumor cells of differentiated neuroblastic tumors was below detection. TFAP2a was strongly expressed in 4 of 6 neuroblastoma cell lines tested, and TFAP2a siRNA mediated knock down in SH-EP cells reduced proliferation and induced a more differentiated phenotype associated with an increase in the expression of the differentiation marker neurotensin.",

author = "Schulte, \{Johannes H.\} and Jutta Kirfel and Soyoung Lim and Alexander Schramm and Nicolaus Friedrichs and Deubzer, \{Hedwig E.\} and Olaf Witt and Angelika Eggert and Reinhard Buettner",

note = "Funding Information: We thank Francoise Halley, Theresia Walter and Birte S{\"o}nnichsen (Cenix BioScience GmbH, Dresden, Germany) for performing the siRNA experiments; Sabine Dreesmann for excellent technical assistance; Kathy Astrahantseff for critical reading of the manuscript as well as Barbara Hero and the German Neuroblastoma Study Group for providing clinical data. A.E., O.W. and A.S. were supported by grants from the National Genome Research Network (NGFN), R.B. was supported by a grant from the DFG. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2008",

month = nov,

day = "18",

doi = "10.1016/j.canlet.2008.05.039",

language = "English",

volume = "271",

pages = "56--63",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

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TY - JOUR

T1 - Transcription factor AP2alpha (TFAP2a) regulates differentiation and proliferation of neuroblastoma cells

AU - Schulte, Johannes H.

AU - Kirfel, Jutta

AU - Lim, Soyoung

AU - Schramm, Alexander

AU - Friedrichs, Nicolaus

AU - Deubzer, Hedwig E.

AU - Witt, Olaf

AU - Eggert, Angelika

AU - Buettner, Reinhard

N1 - Funding Information: We thank Francoise Halley, Theresia Walter and Birte Sönnichsen (Cenix BioScience GmbH, Dresden, Germany) for performing the siRNA experiments; Sabine Dreesmann for excellent technical assistance; Kathy Astrahantseff for critical reading of the manuscript as well as Barbara Hero and the German Neuroblastoma Study Group for providing clinical data. A.E., O.W. and A.S. were supported by grants from the National Genome Research Network (NGFN), R.B. was supported by a grant from the DFG. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2008/11/18

Y1 - 2008/11/18

N2 - Neuroblastoma, the most common extracranial solid tumour of childhood, is derived from neural crest progenitor cells. The TFAP2a transcription factor regulates neural crest patterning. We analysed TFAP2a protein expression in 97 primary neuroblastic tumors and report that TFAP2a was strongly expressed in poorly differentiated neuroblastomas. TFAP2a expression in tumor cells of differentiated neuroblastic tumors was below detection. TFAP2a was strongly expressed in 4 of 6 neuroblastoma cell lines tested, and TFAP2a siRNA mediated knock down in SH-EP cells reduced proliferation and induced a more differentiated phenotype associated with an increase in the expression of the differentiation marker neurotensin.

AB - Neuroblastoma, the most common extracranial solid tumour of childhood, is derived from neural crest progenitor cells. The TFAP2a transcription factor regulates neural crest patterning. We analysed TFAP2a protein expression in 97 primary neuroblastic tumors and report that TFAP2a was strongly expressed in poorly differentiated neuroblastomas. TFAP2a expression in tumor cells of differentiated neuroblastic tumors was below detection. TFAP2a was strongly expressed in 4 of 6 neuroblastoma cell lines tested, and TFAP2a siRNA mediated knock down in SH-EP cells reduced proliferation and induced a more differentiated phenotype associated with an increase in the expression of the differentiation marker neurotensin.

UR - https://www.scopus.com/pages/publications/53249087909

U2 - 10.1016/j.canlet.2008.05.039

DO - 10.1016/j.canlet.2008.05.039

M3 - Journal articles

C2 - 18620802

AN - SCOPUS:53249087909

SN - 0304-3835

VL - 271

SP - 56

EP - 63

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Transcription factor AP2alpha (TFAP2a) regulates differentiation and proliferation of neuroblastoma cells

Abstract

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