Towards a Precise NMR Quantification of Acute Phase Inflammation Proteins from Human Serum

Alvaro Mallagaray*, Lorena Rudolph, Melissa Lindloge, Jarne Mölbitz, Henrik Thomsen, Franziska Schmelter, Mohamad Ward Alhabash, Mohammed R. Abdullah, Roza Saraei, Marc Ehlers, Tobias Graf, Christian Sina, Astrid Petersmann, Matthias Nauck, Ulrich L. Günther*

*Korrespondierende/r Autor/-in für diese Arbeit

Abstract

Nuclear Magnetic Resonance (NMR) spectra of human serum and plasma show, besides metabolites and lipoproteins, two characteristic signals termed GlycA and B arising from the acetyl groups of glycoprotein glycans from acute phase proteins, which constitute good markers for inflammatory processes. Here, we report a comprehensive assignment of glycoprotein glycan NMR signals observed in human serum, showing that GlycA and GlycB signals originate from Neu5Ac and GlcNAc moieties from N-glycans, respectively. Diffusion-edited NMR experiments demonstrate that signal components can be associated with specific acute phase proteins. Conventionally determined concentrations of acute phase glycoproteins correlate well with distinct features in NMR spectra (R2 up to 0.9422, p-value <0.001), allowing the simultaneous quantification of several acute phase inflammation proteins. Overall, a proteo-metabolomics NMR signature of significant diagnostic potential is obtained within 10–20 min acquisition time. This is exemplified in serum samples from COVID-19 and cardiogenic shock patients showing significant changes in several acute phase proteins compared to healthy controls.

OriginalspracheEnglisch
Aufsatznummere202306154
ZeitschriftAngewandte Chemie - International Edition
Jahrgang62
Ausgabenummer35
Seiten (von - bis)e202306154
ISSN1433-7851
DOIs
PublikationsstatusVeröffentlicht - 28.08.2023

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)
  • Zentren: Universitäres Herzzentrum Lübeck (UHZL)

DFG-Fachsystematik

  • 204-05 Immunologie

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