The Protective Efects of Policosanol on Learning and Memory Impairments in a Male Rat Model of Alzheimer’s Disease

Samaneh Safari, Naser Mirazi, Nesa Ahmadi, Masoumeh Asadbegi, Alireza Nourian, Shahab Ghaderi*, · Masome Rashno, Department Neuroscience, School of Science and Advanced Technologies in Medicine, Hamadan University of Medical Sciences, Hamadan, Iran*

*Korrespondierende/r Autor/-in für diese Arbeit


Alzheimer’s disease (AD), the most common form of dementia, is characterized by a progressive decline in cognitive
performance and memory formation. The present study was designed to investigate the efect of policosanol (PCO) on
cognitive function, oxidative-antioxidative status, and amyloid-beta (Aβ) plaque formation in an AD rat model induced
by intracerebroventricular (ICV) injection of Aβ1–40. Healthy adult male Wistar rats were randomly divided into seven
groups: control, sham (5 μL, ICV injection of phosphate-bufered saline), AD model (5 μL, ICV injection of Aβ), acacia
gum (50 mg/kg, 8 weeks, gavage), PCO (50 mg/kg, 8 weeks, gavage), AD+acacia gum (50 mg/kg, 8 weeks, gavage), and
AD+PCO (50 mg/kg, 8 weeks, gavage). During the ninth and tenth weeks of the study, the cognitive function of the rats
was assessed by commonly used behavioral paradigms. Subsequently, oxidative-antioxidative status was examined in the
serum. Moreover, compact Aβ plaques were detected by Congo red staining. The results showed that injection of Aβ impaired
recognition memory in the novel object recognition test, reduced the spatial cognitive ability in the Morris water maze, and
alleviated retention and recall capability in the passive avoidance task. Additionally, injection of Aβ resulted in increased
total oxidant status, decreased total antioxidant capacity, and enhanced Aβ plaque formation in the rats. Intriguingly, PCO
treatment improved all the above-mentioned neuropathological changes in the Aβ-induced AD rats. The results suggest that
PCO improves Aβ-induced cognitive decline, possibly through modulation of oxidative-antioxidative status and inhibition
of Aβ plaque formation.
ZeitschriftMolecular Neurobiology
Seiten (von - bis)2507-2519
PublikationsstatusVeröffentlicht - 05.2023

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)