The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: Target proteins identified

Reinhard Depping*, Christiane Lohaus, Helmut E. Meyer, Wolfgang Rüger

*Korrespondierende/r Autor/-in für diese Arbeit
25 Zitate (Scopus)

Abstract

Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total ∼27) and ModB (total ∼8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.

OriginalspracheEnglisch
ZeitschriftBiochemical and Biophysical Research Communications
Jahrgang335
Ausgabenummer4
Seiten (von - bis)1217-1223
Seitenumfang7
ISSN0006-291X
DOIs
PublikationsstatusVeröffentlicht - 07.10.2005

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)

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