The effect of anakinra on retinal function in isolated perfused vertebrate retina

Mahdy Ranjbar; Toni Schneider; Carl Brand; Salvatore Grisanti; Julia Lüke; Matthias Lüke

doi:10.1016/j.joco.2016.12.002

The effect of anakinra on retinal function in isolated perfused vertebrate retina

Mahdy Ranjbar, Toni Schneider, Carl Brand, Salvatore Grisanti, Julia Lüke, Matthias Lüke

Klinik für Augenheilkunde

Universität zu Köln

4 Zitate (Scopus)

Abstract

PURPOSE: Blockage of the interleukin 1 (IL-1) signaling pathway has been proposed for treatment of inflammatory disorders like those affecting the retina and its adjacent tissue. Herein, we evaluated one of those inhibitory drugs, anakinra (Kineret®), based on its safety profile with emphasis on retinal function from an electrophysiological point of view.

METHODS: Bovine retina preparations were perfused with two different concentrations of anakinra (1 mg/ml and 2 mg/ml). An electroretinogram (ERG) was recorded and b-wave recovery assessed.

RESULTS: Exposure to anakinra at a concentration of 1 mg/ml did not decrease the b-wave amplitude, whereas 2 mg/ml resulted in a significant reduction.

CONCLUSIONS: Based on these preliminary results, anakinra at a dose as low as 1 mg/ml could be regarded as safe for retinal function. However, dosages of 2 mg/ml and more do have toxic electrophysiological effects, at least for the short-term.

Originalsprache	Englisch
Zeitschrift	Journal of current ophthalmology
Jahrgang	29
Ausgabenummer	1
Seiten (von - bis)	69-71
Seitenumfang	3
ISSN	2452-2325
DOIs	https://doi.org/10.1016/j.joco.2016.12.002
Publikationsstatus	Veröffentlicht - 2017

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10.1016/j.joco.2016.12.002

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title = "The effect of anakinra on retinal function in isolated perfused vertebrate retina",

abstract = "PURPOSE: Blockage of the interleukin 1 (IL-1) signaling pathway has been proposed for treatment of inflammatory disorders like those affecting the retina and its adjacent tissue. Herein, we evaluated one of those inhibitory drugs, anakinra (Kineret{\textregistered}), based on its safety profile with emphasis on retinal function from an electrophysiological point of view.METHODS: Bovine retina preparations were perfused with two different concentrations of anakinra (1 mg/ml and 2 mg/ml). An electroretinogram (ERG) was recorded and b-wave recovery assessed.RESULTS: Exposure to anakinra at a concentration of 1 mg/ml did not decrease the b-wave amplitude, whereas 2 mg/ml resulted in a significant reduction.CONCLUSIONS: Based on these preliminary results, anakinra at a dose as low as 1 mg/ml could be regarded as safe for retinal function. However, dosages of 2 mg/ml and more do have toxic electrophysiological effects, at least for the short-term.",

author = "Mahdy Ranjbar and Toni Schneider and Carl Brand and Salvatore Grisanti and Julia L{\"u}ke and Matthias L{\"u}ke",

year = "2017",

doi = "10.1016/j.joco.2016.12.002",

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T1 - The effect of anakinra on retinal function in isolated perfused vertebrate retina

AU - Ranjbar, Mahdy

AU - Schneider, Toni

AU - Brand, Carl

AU - Grisanti, Salvatore

AU - Lüke, Julia

AU - Lüke, Matthias

PY - 2017

Y1 - 2017

N2 - PURPOSE: Blockage of the interleukin 1 (IL-1) signaling pathway has been proposed for treatment of inflammatory disorders like those affecting the retina and its adjacent tissue. Herein, we evaluated one of those inhibitory drugs, anakinra (Kineret®), based on its safety profile with emphasis on retinal function from an electrophysiological point of view.METHODS: Bovine retina preparations were perfused with two different concentrations of anakinra (1 mg/ml and 2 mg/ml). An electroretinogram (ERG) was recorded and b-wave recovery assessed.RESULTS: Exposure to anakinra at a concentration of 1 mg/ml did not decrease the b-wave amplitude, whereas 2 mg/ml resulted in a significant reduction.CONCLUSIONS: Based on these preliminary results, anakinra at a dose as low as 1 mg/ml could be regarded as safe for retinal function. However, dosages of 2 mg/ml and more do have toxic electrophysiological effects, at least for the short-term.

AB - PURPOSE: Blockage of the interleukin 1 (IL-1) signaling pathway has been proposed for treatment of inflammatory disorders like those affecting the retina and its adjacent tissue. Herein, we evaluated one of those inhibitory drugs, anakinra (Kineret®), based on its safety profile with emphasis on retinal function from an electrophysiological point of view.METHODS: Bovine retina preparations were perfused with two different concentrations of anakinra (1 mg/ml and 2 mg/ml). An electroretinogram (ERG) was recorded and b-wave recovery assessed.RESULTS: Exposure to anakinra at a concentration of 1 mg/ml did not decrease the b-wave amplitude, whereas 2 mg/ml resulted in a significant reduction.CONCLUSIONS: Based on these preliminary results, anakinra at a dose as low as 1 mg/ml could be regarded as safe for retinal function. However, dosages of 2 mg/ml and more do have toxic electrophysiological effects, at least for the short-term.

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