The control of pericellular pO2 in monolayer cell cultures: Microelectrode pO2 measurements in human hepatoma cell cultures on gas- permeable and impermeable supports

M. Wolff; J. Fandrey; J. Kalff; A. Muller; W. Jelkmann

The control of pericellular pO₂ in monolayer cell cultures: Microelectrode pO₂ measurements in human hepatoma cell cultures on gas- permeable and impermeable supports

M. Wolff^*, J. Fandrey, J. Kalff, A. Muller, W. Jelkmann

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Physiologie

Rheinische Friedrich-Wilhelms-Universität Bonn

Abstract

The control of pericellular pO₂ is an essential task in cell culture studies concerning cell injury processes, e.g. hypoxia and reoxygenation. Our pO₂ measurements in human hepatoma cultures demonstrate that stationary monolayers may be subjected to severe hypoxia even when kept under conventional room air conditions. This hypoxia develops within 1 h and can be attributed to a misproportion between a high O₂ consumption and a diffusion- limited O₂ supply. The use of gas-permeable dishes provides a close correlation between ambient gas and pericellular O₂ tensions.

Originalsprache	Englisch
Zeitschrift	Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft
Jahrgang	6
Ausgabenummer	2
Seiten (von - bis)	159-161
Seitenumfang	3
ISSN	0935-1965
Publikationsstatus	Veröffentlicht - 1994

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

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title = "The control of pericellular pO2 in monolayer cell cultures: Microelectrode pO2 measurements in human hepatoma cell cultures on gas- permeable and impermeable supports",

abstract = "The control of pericellular pO2 is an essential task in cell culture studies concerning cell injury processes, e.g. hypoxia and reoxygenation. Our pO2 measurements in human hepatoma cultures demonstrate that stationary monolayers may be subjected to severe hypoxia even when kept under conventional room air conditions. This hypoxia develops within 1 h and can be attributed to a misproportion between a high O2 consumption and a diffusion- limited O2 supply. The use of gas-permeable dishes provides a close correlation between ambient gas and pericellular O2 tensions.",

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year = "1994",

language = "English",

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The control of pericellular pO₂ in monolayer cell cultures: Microelectrode pO₂ measurements in human hepatoma cell cultures on gas- permeable and impermeable supports. / Wolff, M.; Fandrey, J.; Kalff, J. et al.
in: Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft, Jahrgang 6, Nr. 2, 1994, S. 159-161.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - The control of pericellular pO2 in monolayer cell cultures: Microelectrode pO2 measurements in human hepatoma cell cultures on gas- permeable and impermeable supports

AU - Wolff, M.

AU - Fandrey, J.

AU - Kalff, J.

AU - Muller, A.

AU - Jelkmann, W.

PY - 1994

Y1 - 1994

N2 - The control of pericellular pO2 is an essential task in cell culture studies concerning cell injury processes, e.g. hypoxia and reoxygenation. Our pO2 measurements in human hepatoma cultures demonstrate that stationary monolayers may be subjected to severe hypoxia even when kept under conventional room air conditions. This hypoxia develops within 1 h and can be attributed to a misproportion between a high O2 consumption and a diffusion- limited O2 supply. The use of gas-permeable dishes provides a close correlation between ambient gas and pericellular O2 tensions.

AB - The control of pericellular pO2 is an essential task in cell culture studies concerning cell injury processes, e.g. hypoxia and reoxygenation. Our pO2 measurements in human hepatoma cultures demonstrate that stationary monolayers may be subjected to severe hypoxia even when kept under conventional room air conditions. This hypoxia develops within 1 h and can be attributed to a misproportion between a high O2 consumption and a diffusion- limited O2 supply. The use of gas-permeable dishes provides a close correlation between ambient gas and pericellular O2 tensions.

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M3 - Journal articles

AN - SCOPUS:0028071499

SN - 0935-1965

VL - 6

SP - 159

EP - 161

JO - Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft

JF - Transplantationsmedizin: Organ der Deutschen Transplantationsgesellschaft

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ER -