Synthesis of Erythropoietins Site-Specifically Conjugated with Complex-Type N-Glycans

Katharina Streichert; Carina Seitz; Eugenia Hoffmann; Irene Boos; Wolfgang Jelkmann; Thomas Brunner; Carlo Unverzagt; Marina Rubini

doi:10.1002/cbic.201900023

Synthesis of Erythropoietins Site-Specifically Conjugated with Complex-Type N-Glycans

Katharina Streichert, Carina Seitz, Eugenia Hoffmann, Irene Boos, Wolfgang Jelkmann, Thomas Brunner, Carlo Unverzagt, Marina Rubini^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Physiologie

12 Zitate (Scopus)

Abstract

The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition. EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding. Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.

Originalsprache	Englisch
Zeitschrift	ChemBioChem
Jahrgang	20
Ausgabenummer	15
Seiten (von - bis)	1914-1918
Seitenumfang	5
ISSN	1439-4227
DOIs	https://doi.org/10.1002/cbic.201900023
Publikationsstatus	Veröffentlicht - 2019

Fördermittel

M.R. and C.U. acknowledge the Deutsche Forschungsgemein-schaft (SPP1623) for financial support. M.R. is grateful to Rainer Winter for providing the CuI complex.

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Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)

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10.1002/cbic.201900023

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title = "Synthesis of Erythropoietins Site-Specifically Conjugated with Complex-Type N-Glycans",

abstract = "The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition. EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding. Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.",

author = "Katharina Streichert and Carina Seitz and Eugenia Hoffmann and Irene Boos and Wolfgang Jelkmann and Thomas Brunner and Carlo Unverzagt and Marina Rubini",

year = "2019",

doi = "10.1002/cbic.201900023",

language = "English",

volume = "20",

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journal = "ChemBioChem",

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T1 - Synthesis of Erythropoietins Site-Specifically Conjugated with Complex-Type N-Glycans

AU - Streichert, Katharina

AU - Seitz, Carina

AU - Hoffmann, Eugenia

AU - Boos, Irene

AU - Jelkmann, Wolfgang

AU - Brunner, Thomas

AU - Unverzagt, Carlo

AU - Rubini, Marina

PY - 2019

Y1 - 2019

N2 - The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition. EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding. Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.

AB - The biological activity of the glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed to readily attach defined N-glycans to EPO through copper-catalyzed azide alkyne cycloaddition. EPO variants with an alkyne-bearing non-natural amino acid (Plk) at the N-glycosylation sites 24, 38, and 83 were obtained by amber suppression followed by protein purification and refolding. Click conjugation of the alkynyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.

UR - https://www.scopus.com/pages/publications/85068710231

U2 - 10.1002/cbic.201900023

DO - 10.1002/cbic.201900023

M3 - Journal articles

C2 - 30973186

AN - SCOPUS:85068710231

SN - 1439-4227

VL - 20

SP - 1914

EP - 1918

JO - ChemBioChem

JF - ChemBioChem

IS - 15

ER -

Synthesis of Erythropoietins Site-Specifically Conjugated with Complex-Type N-Glycans

Abstract

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