Surfactant protein A differentially regulates IFN-γ- and LPS-induced nitrite production by rat alveolar macrophages

C. Stamme, E. Walsh, J. R. Wright*

*Korrespondierende/r Autor/-in für diese Arbeit
51 Zitate (Scopus)

Abstract

Although several studies have demonstrated that the pulmonary collectins surfactant protein (SP)-A and SP-D contribute to innate immunity by enhancing pathogen phagocytosis, the role of SP-A and SP-D in regulating production of free radicals and cytokines is controversial. We hypothesized that the state and mechanism of activation of the immune cell influence its response to SP-A. The effects of SP-A and SP-D on production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) were assessed in isolated rat alveolar macrophages activated with lipopolysaccharide (LPS), interferon gamma (IFN-γ), or both agonists. SP-A inhibited production of NO and iNOS in macrophages stimulated with smooth LPS, which did not significantly bind SP-A, or rough LPS, which avidly bound SP-A. In contrast, SP-A enhanced production of NO and iNOS in cells stimulated with IFN-γ or INF-γ plus LPS. Neither SP-A nor SP-D affected baseline NO production, and SP-D did not significantly affect production of NO in cells stimulated with either LPS or IFN-γ. These results suggest that SP-A contributes to the lung inflammatory response by exerting differential effects on the responses of immune cells, depending on their state and mechanism of activation.

OriginalspracheEnglisch
ZeitschriftAmerican Journal of Respiratory Cell and Molecular Biology
Jahrgang23
Ausgabenummer6
Seiten (von - bis)772-779
Seitenumfang8
ISSN1044-1549
DOIs
PublikationsstatusVeröffentlicht - 2000

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