Streptococcus pneumoniae-induced regulation of cyclooxygenase-2 in human lung tissue

Kolja V. Szymanski, Mario Toennies, Anne Becher, Diana Fatykhova, Philippe D. N'Guessan, Birgitt Gutbier, Frederick Klauschen, Frank Neuschaefer-Rube, Paul Schneider, Jens Rueckert, Jens Neudecker, Torsten T. Bauer, Klaus Dalhoff, Daniel Drom̈ann, Achim D. Gruber, Olivia Kershaw, Bettina Temmesfeld-Wollbrueck, Norbert Suttorp, Stefan Hippenstiel, Andreas C. Hocke*

*Korrespondierende/r Autor/-in für diese Arbeit
19 Zitate (Scopus)

Abstract

The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue. Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites. In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid4receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies, cyclooxygenase-2/prostaglandin E2 related pro- and anti-inflammatory mediator regulation was partly confirmed in human lung tissue after pneumococcal infection. Overall, cell type-specific and MAPK-dependent cyclooxygenase-2 expression and prostaglandin E2 formation in human lung tissue may play an important role in the early phase of pneumococcal infections. Copyright

OriginalspracheEnglisch
ZeitschriftEuropean Respiratory Journal
Jahrgang40
Ausgabenummer6
Seiten (von - bis)1458-1467
Seitenumfang10
ISSN0903-1936
DOIs
PublikationsstatusVeröffentlicht - 01.12.2012

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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