Abstract
In vitro maturation (IVM) is a novel approach to overcome the adverse effects of human in vitro fertilization (IVF). The aim of the present study is to evaluate the effect of total and steroid-depleted serum obtained from patients with endometriosis on IVM outcome as supplementation for this system. To this purpose, patients with endometriosis were selected according to in/excluding criteria. Germinal vesicles (GVs) and cumulus cells were treated with 10% of each serum. The expression levels of stearoyl CoA desaturase 1 (SCD 1) and cyclooxygenase-2 (COX-2) genes were evaluated by RT-qPCR. Gas-liquid chromatography and flow cytometry were performed to analyze fatty acids composition and apoptosis. The mRNA expression levels of SCD1 (2.47 fold) and COX-2 (6.4 fold), and also the synthesis of oleate, linoleate, and arachidonate were increased (1.19, 1.06, and 2.37 folds, respectively) in cumulus cells treated with steroid-depleted serum (p <.05). The synthesis of palmitate, palmitoleate, and stearate (0.995, 0.67, and 0.7 folds, respectively) and also the rate of apoptosis were significantly decreased in these cells (p <.05). Moreover, GVs cultured in steroid-depleted group showed a significantly higher rate of maturation (p <.001). Overall, our findings imply a new insight into the expansion of IVM system in oocytes development.
| Originalsprache | Englisch |
|---|---|
| Zeitschrift | Journal of Cellular Physiology |
| Jahrgang | 236 |
| Ausgabenummer | 1 |
| Seiten (von - bis) | 205-214 |
| Seitenumfang | 10 |
| ISSN | 0021-9541 |
| DOIs | |
| Publikationsstatus | Veröffentlicht - 01.2021 |
Fördermittel
The authors are grateful of the department of Advanced Medical Sciences, Tabriz University of Medical Sciences for supporting this study. This paper is the outcome of a master thesis of medical biotechnology (thesis no. 93/2-10/7) approved in the Department of Advanced Medical Sciences at Tabriz University of Medical Sciences and is supported by a grant from this center. The authors are grateful of the department of Advanced Medical Sciences, Tabriz University of Medical Sciences for supporting this study. This paper is the outcome of a master thesis of medical biotechnology (thesis no. 93/2‐10/7) approved in the Department of Advanced Medical Sciences at Tabriz University of Medical Sciences and is supported by a grant from this center.
UN SDGs
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