Steroid-depleted endometriosis serum improves oocyte maturation in IVM systems

Samira Asghari, Mohammad Nouri, Masoud Darabi, Amir Valizadeh*

*Korrespondierende/r Autor/-in für diese Arbeit
2 Zitate (Scopus)


In vitro maturation (IVM) is a novel approach to overcome the adverse effects of human in vitro fertilization (IVF). The aim of the present study is to evaluate the effect of total and steroid-depleted serum obtained from patients with endometriosis on IVM outcome as supplementation for this system. To this purpose, patients with endometriosis were selected according to in/excluding criteria. Germinal vesicles (GVs) and cumulus cells were treated with 10% of each serum. The expression levels of stearoyl CoA desaturase 1 (SCD 1) and cyclooxygenase-2 (COX-2) genes were evaluated by RT-qPCR. Gas-liquid chromatography and flow cytometry were performed to analyze fatty acids composition and apoptosis. The mRNA expression levels of SCD1 (2.47 fold) and COX-2 (6.4 fold), and also the synthesis of oleate, linoleate, and arachidonate were increased (1.19, 1.06, and 2.37 folds, respectively) in cumulus cells treated with steroid-depleted serum (p <.05). The synthesis of palmitate, palmitoleate, and stearate (0.995, 0.67, and 0.7 folds, respectively) and also the rate of apoptosis were significantly decreased in these cells (p <.05). Moreover, GVs cultured in steroid-depleted group showed a significantly higher rate of maturation (p <.001). Overall, our findings imply a new insight into the expansion of IVM system in oocytes development.

ZeitschriftJournal of Cellular Physiology
Seiten (von - bis)205-214
PublikationsstatusVeröffentlicht - 01.2021