TY - JOUR
T1 - Specific inhibition of human immunodeficiency virus type 1 replication by RNA transcribed in sense and antisense orientation from the 5′-leader/gag region
AU - Sczakiel, Georg
AU - PAWLITA, MICHAEL
AU - Kleinheinz, Andreas
N1 - Funding Information:
We thank H. xur Hausen and L. Giimann for stimulating discussions and continuous encouragement. We also thank M. Martin for providing HIV-l proviral clone pNL4-3, R.C. GaIIo for HIV-l clone pBH10, H.-P. Vosberg for plasmid pHIV410 and H. Stijppler for plasmid pCMV-CAT. We thank A. Henn and H. G6txmann for excellent technical assistance and V. Bosch and H.-G. Rrgusslich for criticaIIy reading this manuscript. This work was supported by BMFf grant FKZ B-083-89.
PY - 1990/6/15
Y1 - 1990/6/15
N2 - The inhibitory effects of expression plasmids on HIV-1 replication were studied in a transient assay system. Test plasmids were co-microinjected with non-defective proviral HIV-1 DNA into a colon-carcinoma cell line (SW480) and the resulting infectious HIV-1 was quantitated after amplification in cocultivated CD4+ MT-4 cells. At a molar ratio of 1:1 and 5:1 plasmids capable of expressing a 410 bp HIV-1 fragment as antisense or sense transcript respectively both specifically inhibited HIV-1 replication up to 70%. This effect was specific for HIV-1 sequences and was not observed upon expression of unrelated RNA-segments. At a molar excess equal to or greater than 15:1, additional inhibitory effects were seen with control plasmids carrying only the strong human cytomegalovirus immediate early (HCMV IE) promoter/enhancer element. The reasons for these findings are discussed.
AB - The inhibitory effects of expression plasmids on HIV-1 replication were studied in a transient assay system. Test plasmids were co-microinjected with non-defective proviral HIV-1 DNA into a colon-carcinoma cell line (SW480) and the resulting infectious HIV-1 was quantitated after amplification in cocultivated CD4+ MT-4 cells. At a molar ratio of 1:1 and 5:1 plasmids capable of expressing a 410 bp HIV-1 fragment as antisense or sense transcript respectively both specifically inhibited HIV-1 replication up to 70%. This effect was specific for HIV-1 sequences and was not observed upon expression of unrelated RNA-segments. At a molar excess equal to or greater than 15:1, additional inhibitory effects were seen with control plasmids carrying only the strong human cytomegalovirus immediate early (HCMV IE) promoter/enhancer element. The reasons for these findings are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0025361871&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)90379-2
DO - 10.1016/0006-291X(90)90379-2
M3 - Journal articles
C2 - 1694076
AN - SCOPUS:0025361871
SN - 0006-291X
VL - 169
SP - 643
EP - 651
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
