Species-Specific Functional Regions of the Green Alga Gamete Fusion Protein HAP2 Revealed by Structural Studies

Eduard Baquero; Juliette Fedry; Pierre Legrand; Thomas Krey; Felix A. Rey

doi:10.1016/j.str.2018.09.014

Species-Specific Functional Regions of the Green Alga Gamete Fusion Protein HAP2 Revealed by Structural Studies

Eduard Baquero, Juliette Fedry, Pierre Legrand, Thomas Krey, Felix A. Rey^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Biochemie

21 Zitate (Scopus)

Abstract

Baquero et al. report the structure of the HAP2 gamete fusion protein of the Chlamydomonas reinhardtii alga, describing key elements required to bridge two opposite-type gametes and drive their fusion. Mutagenesis of these elements confirmed their membrane-interaction function. Additional specific HAP2 protein-protein interaction sites are described as potential fusion controls.

Originalsprache	Englisch
Zeitschrift	Structure
Jahrgang	27
Ausgabenummer	1
Seiten (von - bis)	113-124.e4
ISSN	0969-2126
DOIs	https://doi.org/10.1016/j.str.2018.09.014
Publikationsstatus	Veröffentlicht - 02.01.2019

Fördermittel

Funding for this project was provided by the European Research Council (ERC) advanced grant CelCelFus (grant number 340371) to F.A.R., as well as recurrent funding from Institut Pasteur and the CNRS. J.F. had a PhD fellowhsip from Ecole Polytechnique AMX. We thank I. Fernandez for technical help and discussion; W.J. Snell for helpful discussions and for testing the effect antibodies against HAP2 on C. reinhardtii gamete fusion in vivo; Ahmed Haouz and staff of the Pasteur crystallogenesis facility as well as the staff of Proxima-1 and -2 at the SOLEIL synchrotron for help during crystallization screening and diffraction data collection. Funding for this project was provided by the European Research Council (ERC) advanced grant CelCelFus (grant number 340371 ) to F.A.R., as well as recurrent funding from Institut Pasteur and the CNRS . J.F. had a PhD fellowhsip from Ecole Polytechnique AMX. We thank I. Fernandez for technical help and discussion; W.J. Snell for helpful discussions and for testing the effect antibodies against HAP2 on C. reinhardtii gamete fusion in vivo; Ahmed Haouz and staff of the Pasteur crystallogenesis facility as well as the staff of Proxima-1 and -2 at the SOLEIL synchrotron for help during crystallization screening and diffraction data collection.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen

Dokumente

10.1016/j.str.2018.09.014

Weitere Links

Link to publication in Scopus

Zitieren

@article{e02242140fe943ab8cb8751485624ff8,

title = "Species-Specific Functional Regions of the Green Alga Gamete Fusion Protein HAP2 Revealed by Structural Studies",

abstract = "Baquero et al. report the structure of the HAP2 gamete fusion protein of the Chlamydomonas reinhardtii alga, describing key elements required to bridge two opposite-type gametes and drive their fusion. Mutagenesis of these elements confirmed their membrane-interaction function. Additional specific HAP2 protein-protein interaction sites are described as potential fusion controls.",

author = "Eduard Baquero and Juliette Fedry and Pierre Legrand and Thomas Krey and Rey, \{Felix A.\}",

note = "Publisher Copyright: {\textcopyright} 2018 The Authors",

year = "2019",

month = jan,

day = "2",

doi = "10.1016/j.str.2018.09.014",

language = "English",

volume = "27",

pages = "113--124.e4",

journal = "Structure",

issn = "0969-2126",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Species-Specific Functional Regions of the Green Alga Gamete Fusion Protein HAP2 Revealed by Structural Studies

AU - Baquero, Eduard

AU - Fedry, Juliette

AU - Legrand, Pierre

AU - Krey, Thomas

AU - Rey, Felix A.

PY - 2019/1/2

Y1 - 2019/1/2

N2 - Baquero et al. report the structure of the HAP2 gamete fusion protein of the Chlamydomonas reinhardtii alga, describing key elements required to bridge two opposite-type gametes and drive their fusion. Mutagenesis of these elements confirmed their membrane-interaction function. Additional specific HAP2 protein-protein interaction sites are described as potential fusion controls.

AB - Baquero et al. report the structure of the HAP2 gamete fusion protein of the Chlamydomonas reinhardtii alga, describing key elements required to bridge two opposite-type gametes and drive their fusion. Mutagenesis of these elements confirmed their membrane-interaction function. Additional specific HAP2 protein-protein interaction sites are described as potential fusion controls.

UR - https://www.scopus.com/pages/publications/85059494283

U2 - 10.1016/j.str.2018.09.014

DO - 10.1016/j.str.2018.09.014

M3 - Journal articles

C2 - 30416037

AN - SCOPUS:85059494283

SN - 0969-2126

VL - 27

SP - 113-124.e4

JO - Structure

JF - Structure

IS - 1

ER -

Species-Specific Functional Regions of the Green Alga Gamete Fusion Protein HAP2 Revealed by Structural Studies

Abstract

Fördermittel

UN SDGs

Dokumente

Weitere Links

Fingerprint

Zitieren