Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate

Matthias Eibl, Sebastian Karpf, Daniel Weng, Hubertus Hakert, Tom Pfeiffer, Jan Philip Kolb, Robert Huber

12 Zitate (Scopus)

Abstract

Two-photon-excited fluorescence lifetime imaging microscopy (FLIM) is a chemically specific 3-D sensing modality providing valuable information about the microstructure, composition and function of a sample. However, a more widespread application of this technique is hindered by the need for a sophisticated ultra-short pulse laser source and by speed limitations of current FLIM detection systems. To overcome these limitations, we combined a robust sub-nanosecond fiber laser as the excitation source with high analog bandwidth detection. Due to the long pulse length in our configuration, more fluorescence photons are generated per pulse, which allows us to derive the lifetime with a single excitation pulse only. In this paper, we show high quality FLIM images acquired at a pixel rate of 1 MHz. This approach is a promising candidate for an easy-to-use and benchtop FLIM system to make this technique available to a wider research community.

OriginalspracheEnglisch
Aufsatznummer288056
ZeitschriftBiomedical Optics Express
Jahrgang8
Ausgabenummer7
Seiten (von - bis)3132-3142
Seitenumfang11
DOIs
PublikationsstatusVeröffentlicht - 01.07.2017

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Biomedizintechnik

Fingerprint

Untersuchen Sie die Forschungsthemen von „Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate“. Zusammen bilden sie einen einzigartigen Fingerprint.

Zitieren