Shikimate Pathway in Non-Photosynthetic Tissues Identification of Common Enzymes and Partial Purification of Dehydroquinate Hydrolyase-Shikimate Oxidoreductase and Chorismate Mutase from Roots

The present study aims to give a survey on common enzymes of the shikimate pathway in non-green tissues. Dehydroquinate hydrolyase/shikimate oxidoreductase, shikimate kinase and chorismate mutase from roots of spinach and a radish (Brassica rapa) hybrid were partially purified; the 3-dehydroquinate synthase was identified. Regarding some earlier findings of other authors and previewing current intermediate incorporation studies, it can be inferred that non-green tissues make use of the complete shikimate pathway to synthesize aromatic amino acids - unlike the pathway in photosynthetic tissues - independent from light.