Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution

Yahya Açil; Jürgen Brinckmann; Peter Behrens; Peter K. Müller; Boris Bätge

doi:10.1016/S0021-9673(96)00729-7

Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution

Yahya Açil^*, Jürgen Brinckmann, Peter Behrens, Peter K. Müller, Boris Bätge

^*Korrespondierende/r Autor/-in für diese Arbeit

13 Zitate (Scopus)

Abstract

A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.

Originalsprache	Englisch
Zeitschrift	Journal of Chromatography A
Jahrgang	758
Ausgabenummer	2
Seiten (von - bis)	313-318
Seitenumfang	6
ISSN	0021-9673
DOIs	https://doi.org/10.1016/S0021-9673(96)00729-7
Publikationsstatus	Veröffentlicht - 17.01.1997

Fördermittel

This work was supportedb y DFG (Deutsche For-schungsgemeinschaSftF;B 367-A1 and Br 1146/2-1), by a grant from the BMFT (Bundesministerium ftir Forschung und Technologie0, 1 KM 9303) and by a grant from the registereds ociety for investigation and fight of rheumatoid diseases (Bad BramstedtG, ermany).

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Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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10.1016/S0021-9673(96)00729-7

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title = "Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution",

abstract = "A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95\%) and purity (>98\%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.",

author = "Yahya A{\c c}il and J{\"u}rgen Brinckmann and Peter Behrens and M{\"u}ller, \{Peter K.\} and Boris B{\"a}tge",

year = "1997",

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doi = "10.1016/S0021-9673(96)00729-7",

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Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution. / Açil, Yahya; Brinckmann, Jürgen; Behrens, Peter et al.
in: Journal of Chromatography A, Jahrgang 758, Nr. 2, 17.01.1997, S. 313-318.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution

AU - Açil, Yahya

AU - Brinckmann, Jürgen

AU - Behrens, Peter

AU - Müller, Peter K.

AU - Bätge, Boris

PY - 1997/1/17

Y1 - 1997/1/17

N2 - A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.

AB - A simple method for the isolation of α-chains of different collagen types was developed. The procedure involves sodium dodecyl sulfatae-polyacrylamide gel electrophoresis followed by electroelution of separated and defixed collagen α-chains. Collagen types I, II, III and V from different porcine tissues were recovered in high quantity (>95%) and purity (>98%) as evidenced by amino acid analysis. The procedure can be used for sample quantities smaller than required for conventional methods, e.g, chromatographic procedures.

UR - https://www.scopus.com/pages/publications/0031575377

U2 - 10.1016/S0021-9673(96)00729-7

DO - 10.1016/S0021-9673(96)00729-7

M3 - Journal articles

C2 - 9042738

AN - SCOPUS:0031575377

SN - 0021-9673

VL - 758

SP - 313

EP - 318

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 2

ER -

Semipreparative isolation of collagen types I, II, III and V by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroelution

Abstract

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