Selecting karyophilic DNA cis elements in Xenopus laevis oocytes; a new approach

Marco Marcello, Roger Fischer, Helmut Tröster, Michael F. Trendelenburg*, Georg Sczakiel

*Korrespondierende/r Autor/-in für diese Arbeit
    2 Zitate (Scopus)


    The intracellular localisation and mobility of exogenous DNA introduced into Xenopus laevis oocytes is largely unknown. In this paper, we report a new technique to investigate the cytoplasmic/nuclear transport of a random pool of linear, double-stranded, oligomeric DNA of 147 bp in length. We chose a combinatorial approach which made use of repetitive rounds of selection and amplification to search for new cis elements mediating nuclear import or retention. A new PCR-based methodology was established to reliably detect exogenous DNA in subcellular and total extracts prepared from Xenopus laevis oocytes. Studies in vivo and with cellular extracts indicate the presence of a highly efficient nuclease activity in the nuclear compartment. The described combinatorial approach constitutes a promising tool for the isolation of novel DNA cis elements which may play an important role in the nuclear internalisation and retention of exogenous DNA in Xenopus laevis oocytes.

    ZeitschriftInternational Journal of Developmental Biology
    Seiten (von - bis)309-316
    PublikationsstatusVeröffentlicht - 22.06.2002


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