RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation

Helga Grötsch; Marlene Kunert; Katrin A. Mooslehner; Zhigang Gao; Dagmar Struve; Ieuan A. Hughes; Olaf Hiort; Ralf Werner

doi:10.1016/j.mce.2012.02.020

RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation

Helga Grötsch, Marlene Kunert, Katrin A. Mooslehner, Zhigang Gao, Dagmar Struve, Ieuan A. Hughes, Olaf Hiort, Ralf Werner^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Klinik für Kinder- und Jugendmedizin

9 Zitate (Scopus)

Abstract

During embryogenesis, the development of the male genital is dependent on androgens. Their actions are mediated by the androgen receptor (AR), which functions as a transcription factor. To identify AR coregulators that support AR action during the critical time window of androgen-dependent development in the genital tubercle of male mice, we performed yeast two-hybrid screenings with cDNA libraries of genital tubercles from male mouse embryos using human AR as bait. RWD domain containing 1 (RWDD1) was identified as an AR-interacting protein from three independent libraries of the embryonic days E15, E16 and E17. The interaction between the AR and RWDD1 was confirmed in vitro and in vivo and the ligand binding domain of the AR was shown to be sufficient to mediate the interaction. RWDD1 enhanced AR-dependent transactivation in reporter assays with promoters of different complexity and in different cell lines. These results suggest that RWDD1 functions as a coactivator of androgen-dependent transcription.

Originalsprache	Englisch
Zeitschrift	Molecular and Cellular Endocrinology
Jahrgang	358
Ausgabenummer	1
Seiten (von - bis)	53-62
Seitenumfang	10
ISSN	0303-7207
DOIs	https://doi.org/10.1016/j.mce.2012.02.020
Publikationsstatus	Veröffentlicht - 06.07.2012

Fördermittel

The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013) under Grant Agreement No. 201444 (EuroDSD). H. Grötsch is a recipient of a Grant from the Medical Faculty of the University of Lübeck, Grant No. E19-2011. We thank H. Merz and A. Dummer from the Department of Pathology of the University of Lübeck for technical support with paraffin-embedding and tissue sample preparation and A.O. Brinkmann, F. Claessens and P.M. Holterhus for providing plasmids. We thank B. Brix for critical reading of the manuscript and M. Szaszak for technical help with the microscope. Microscopy equipment was funded by a Grant from the Medical Faculty of the University of Lübeck, Grant No. E01-2011.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen
SDG 5 – Gender Equality
SDG 10 – Weniger Ungleichheiten

Dokumente

10.1016/j.mce.2012.02.020

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@article{d82be6b0d083489786303994df0fcfa5,

title = "RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation",

abstract = "During embryogenesis, the development of the male genital is dependent on androgens. Their actions are mediated by the androgen receptor (AR), which functions as a transcription factor. To identify AR coregulators that support AR action during the critical time window of androgen-dependent development in the genital tubercle of male mice, we performed yeast two-hybrid screenings with cDNA libraries of genital tubercles from male mouse embryos using human AR as bait. RWD domain containing 1 (RWDD1) was identified as an AR-interacting protein from three independent libraries of the embryonic days E15, E16 and E17. The interaction between the AR and RWDD1 was confirmed in vitro and in vivo and the ligand binding domain of the AR was shown to be sufficient to mediate the interaction. RWDD1 enhanced AR-dependent transactivation in reporter assays with promoters of different complexity and in different cell lines. These results suggest that RWDD1 functions as a coactivator of androgen-dependent transcription.",

author = "Helga Gr{\"o}tsch and Marlene Kunert and Mooslehner, \{Katrin A.\} and Zhigang Gao and Dagmar Struve and Hughes, \{Ieuan A.\} and Olaf Hiort and Ralf Werner",

note = "Funding Information: The research leading to these results has received funding from the European Community{\textquoteright}s Seventh Framework Programme (FP7/2007–2013) under Grant Agreement No. 201444 (EuroDSD). H. Gr{\"o}tsch is a recipient of a Grant from the Medical Faculty of the University of L{\"u}beck, Grant No. E19-2011. Funding Information: We thank H. Merz and A. Dummer from the Department of Pathology of the University of L{\"u}beck for technical support with paraffin-embedding and tissue sample preparation and A.O. Brinkmann, F. Claessens and P.M. Holterhus for providing plasmids. We thank B. Brix for critical reading of the manuscript and M. Szaszak for technical help with the microscope. Microscopy equipment was funded by a Grant from the Medical Faculty of the University of L{\"u}beck, Grant No. E01-2011. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2012",

month = jul,

day = "6",

doi = "10.1016/j.mce.2012.02.020",

language = "English",

volume = "358",

pages = "53--62",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation. / Grötsch, Helga; Kunert, Marlene; Mooslehner, Katrin A. et al.
in: Molecular and Cellular Endocrinology, Jahrgang 358, Nr. 1, 06.07.2012, S. 53-62.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation

AU - Grötsch, Helga

AU - Kunert, Marlene

AU - Mooslehner, Katrin A.

AU - Gao, Zhigang

AU - Struve, Dagmar

AU - Hughes, Ieuan A.

AU - Hiort, Olaf

AU - Werner, Ralf

N1 - Funding Information: The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013) under Grant Agreement No. 201444 (EuroDSD). H. Grötsch is a recipient of a Grant from the Medical Faculty of the University of Lübeck, Grant No. E19-2011. Funding Information: We thank H. Merz and A. Dummer from the Department of Pathology of the University of Lübeck for technical support with paraffin-embedding and tissue sample preparation and A.O. Brinkmann, F. Claessens and P.M. Holterhus for providing plasmids. We thank B. Brix for critical reading of the manuscript and M. Szaszak for technical help with the microscope. Microscopy equipment was funded by a Grant from the Medical Faculty of the University of Lübeck, Grant No. E01-2011. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012/7/6

Y1 - 2012/7/6

N2 - During embryogenesis, the development of the male genital is dependent on androgens. Their actions are mediated by the androgen receptor (AR), which functions as a transcription factor. To identify AR coregulators that support AR action during the critical time window of androgen-dependent development in the genital tubercle of male mice, we performed yeast two-hybrid screenings with cDNA libraries of genital tubercles from male mouse embryos using human AR as bait. RWD domain containing 1 (RWDD1) was identified as an AR-interacting protein from three independent libraries of the embryonic days E15, E16 and E17. The interaction between the AR and RWDD1 was confirmed in vitro and in vivo and the ligand binding domain of the AR was shown to be sufficient to mediate the interaction. RWDD1 enhanced AR-dependent transactivation in reporter assays with promoters of different complexity and in different cell lines. These results suggest that RWDD1 functions as a coactivator of androgen-dependent transcription.

AB - During embryogenesis, the development of the male genital is dependent on androgens. Their actions are mediated by the androgen receptor (AR), which functions as a transcription factor. To identify AR coregulators that support AR action during the critical time window of androgen-dependent development in the genital tubercle of male mice, we performed yeast two-hybrid screenings with cDNA libraries of genital tubercles from male mouse embryos using human AR as bait. RWD domain containing 1 (RWDD1) was identified as an AR-interacting protein from three independent libraries of the embryonic days E15, E16 and E17. The interaction between the AR and RWDD1 was confirmed in vitro and in vivo and the ligand binding domain of the AR was shown to be sufficient to mediate the interaction. RWDD1 enhanced AR-dependent transactivation in reporter assays with promoters of different complexity and in different cell lines. These results suggest that RWDD1 functions as a coactivator of androgen-dependent transcription.

UR - https://www.scopus.com/pages/publications/84860459265

U2 - 10.1016/j.mce.2012.02.020

DO - 10.1016/j.mce.2012.02.020

M3 - Journal articles

C2 - 22406838

AN - SCOPUS:84860459265

SN - 0303-7207

VL - 358

SP - 53

EP - 62

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 1

ER -

RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation

Abstract

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