Role of cytochrome P450 in the control of the production of erythropoietin

J. Fandrey; F. P. Seydel; C. P. Siegers; W. Jelkmann

doi:10.1016/0024-3205(90)90225-G

Role of cytochrome P₄₅₀ in the control of the production of erythropoietin

J. Fandrey^*, F. P. Seydel, C. P. Siegers, W. Jelkmann

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Physiologie

49 Zitate (Scopus)

Abstract

Effects of agents affecting cytochrome P_{4 5 0} were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 H of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P_{4 5 0}, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T₃ and T₄ stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P_{4 5 0}. These findings support the idea that O₂ sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.

Originalsprache	Englisch
Zeitschrift	Life Sciences
Jahrgang	47
Ausgabenummer	2
Seiten (von - bis)	127-134
Seitenumfang	8
ISSN	0024-3205
DOIs	https://doi.org/10.1016/0024-3205(90)90225-G
Publikationsstatus	Veröffentlicht - 1990

Fördermittel

The authors gratefully acknowledge the expert technical assistance of Miss Sabine GieSelmann and the secreterial work of Mrs. Gisela Thaler. This work was financially supported by the Deutsche Forschungsgemeinschaft (DFG-grant Je 95/6-2).

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Gehirn, Hormone, Verhalten - Center for Brain, Behavior and Metabolism (CBBM)

Dokumente

10.1016/0024-3205(90)90225-G

Weitere Links

Link to publication in Scopus

Zitieren

@article{0c3d69b1eb9449488fe0799fb00e2180,

title = "Role of cytochrome P450 in the control of the production of erythropoietin",

abstract = "Effects of agents affecting cytochrome P4 5 0 were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 H of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P4 5 0, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T3 and T4 stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P4 5 0. These findings support the idea that O2 sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.",

author = "J. Fandrey and Seydel, \{F. P.\} and Siegers, \{C. P.\} and W. Jelkmann",

note = "Funding Information: The authors gratefully acknowledge the expert technical assistance of Miss Sabine GieSelmann and the secreterial work of Mrs. Gisela Thaler. This work was financially supported by the Deutsche Forschungsgemeinschaft (DFG-grant Je 95/6-2). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1990",

doi = "10.1016/0024-3205(90)90225-G",

language = "English",

volume = "47",

pages = "127--134",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Role of cytochrome P450 in the control of the production of erythropoietin

AU - Fandrey, J.

AU - Seydel, F. P.

AU - Siegers, C. P.

AU - Jelkmann, W.

N1 - Funding Information: The authors gratefully acknowledge the expert technical assistance of Miss Sabine GieSelmann and the secreterial work of Mrs. Gisela Thaler. This work was financially supported by the Deutsche Forschungsgemeinschaft (DFG-grant Je 95/6-2). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1990

Y1 - 1990

N2 - Effects of agents affecting cytochrome P4 5 0 were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 H of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P4 5 0, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T3 and T4 stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P4 5 0. These findings support the idea that O2 sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.

AB - Effects of agents affecting cytochrome P4 5 0 were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 H of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P4 5 0, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T3 and T4 stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P4 5 0. These findings support the idea that O2 sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.

UR - https://www.scopus.com/pages/publications/0025193093

U2 - 10.1016/0024-3205(90)90225-G

DO - 10.1016/0024-3205(90)90225-G

M3 - Journal articles

C2 - 2167416

AN - SCOPUS:0025193093

SN - 0024-3205

VL - 47

SP - 127

EP - 134

JO - Life Sciences

JF - Life Sciences

IS - 2

ER -