Requisite instruments for the establishment of three-dimensional epidermal human skin equivalents—A methods review

Christianna Marie Czyz; Paul Werner Kunth; Florian Gruber; Christopher Kremslehner; Christoph Matthias Hammers; Jennifer Elisabeth Hundt

doi:10.1111/exd.14911

Requisite instruments for the establishment of three-dimensional epidermal human skin equivalents—A methods review

Christianna Marie Czyz^*, Paul Werner Kunth^*, Florian Gruber, Christopher Kremslehner, Christoph Matthias Hammers, Jennifer Elisabeth Hundt

^*Korrespondierende/r Autor/-in für diese Arbeit

University of Vienna

8 Zitate (Scopus)

Abstract

Human skin equivalents (HSEs) are three-dimensional skin organ culture models raised in vitro. This review gives an overview of common techniques for setting up HSEs. The HSE consists of an artificial dermis and epidermis. 3T3-J2 murine fibroblasts, purchased human fibroblasts or freshly isolated and cultured fibroblasts, together with other components, for example, collagen type I, are used to build the scaffold. Freshly isolated and cultured keratinocytes are seeded on top. It is possible to add other cell types, for example, melanocytes, to the HSE—depending on the research question. After several days and further steps, the 3D skin can be harvested. Additionally, we show possible markers and techniques for evaluation of artificial skin. Furthermore, we provide a comparison of HSEs to human skin organ culture, a model which employs human donor skin. We outline advantages and limitations of both models and discuss future perspectives in using HSEs.

Originalsprache	Englisch
Zeitschrift	Experimental Dermatology
Jahrgang	32
Ausgabenummer	11
Seiten (von - bis)	1870-1883
Seitenumfang	14
ISSN	0906-6705
DOIs	https://doi.org/10.1111/exd.14911
Publikationsstatus	Veröffentlicht - 11.2023

Fördermittel

This review was supported by DFG funding for the Collaborative Research Center (CRC) 1526 – Pathomechanisms of Antibody‐mediated Autoimmunity (PANTAU) – Insights from Pemphigoid Diseases (Grant No.: 454193335) to CMH. Additionally the research was supported by DFG funding for the Research Training Group (RTG) 1727 – Modulation of Autoimmunity (Project number 179309734) to CMC and PWK. Open Access funding enabled and organized by Projekt DEAL.

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

SDG 3 – Gesundheit und Wohlergehen
SDG 9 – Industrie, Innovation und Infrastruktur

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)
Zentren: Center for Research on Inflammation of the Skin (CRIS)
Forschungsschwerpunkt: Biomedizintechnik

DFG-Fachsystematik

2.22-19 Dermatologie
2.22-32 Medizinische Physik, Biomedizinische Technik
2.22-07 Medizininformatik und medizinische Bioinformatik

Dokumente

10.1111/exd.14911

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title = "Requisite instruments for the establishment of three-dimensional epidermal human skin equivalents—A methods review",

abstract = "Human skin equivalents (HSEs) are three-dimensional skin organ culture models raised in vitro. This review gives an overview of common techniques for setting up HSEs. The HSE consists of an artificial dermis and epidermis. 3T3-J2 murine fibroblasts, purchased human fibroblasts or freshly isolated and cultured fibroblasts, together with other components, for example, collagen type I, are used to build the scaffold. Freshly isolated and cultured keratinocytes are seeded on top. It is possible to add other cell types, for example, melanocytes, to the HSE—depending on the research question. After several days and further steps, the 3D skin can be harvested. Additionally, we show possible markers and techniques for evaluation of artificial skin. Furthermore, we provide a comparison of HSEs to human skin organ culture, a model which employs human donor skin. We outline advantages and limitations of both models and discuss future perspectives in using HSEs.",

author = "Czyz, \{Christianna Marie\} and Kunth, \{Paul Werner\} and Florian Gruber and Christopher Kremslehner and Hammers, \{Christoph Matthias\} and Hundt, \{Jennifer Elisabeth\}",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors. Experimental Dermatology published by John Wiley \& Sons Ltd.",

year = "2023",

month = nov,

doi = "10.1111/exd.14911",

language = "English",

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AU - Czyz, Christianna Marie

AU - Kunth, Paul Werner

AU - Gruber, Florian

AU - Kremslehner, Christopher

AU - Hammers, Christoph Matthias

AU - Hundt, Jennifer Elisabeth

PY - 2023/11

Y1 - 2023/11

N2 - Human skin equivalents (HSEs) are three-dimensional skin organ culture models raised in vitro. This review gives an overview of common techniques for setting up HSEs. The HSE consists of an artificial dermis and epidermis. 3T3-J2 murine fibroblasts, purchased human fibroblasts or freshly isolated and cultured fibroblasts, together with other components, for example, collagen type I, are used to build the scaffold. Freshly isolated and cultured keratinocytes are seeded on top. It is possible to add other cell types, for example, melanocytes, to the HSE—depending on the research question. After several days and further steps, the 3D skin can be harvested. Additionally, we show possible markers and techniques for evaluation of artificial skin. Furthermore, we provide a comparison of HSEs to human skin organ culture, a model which employs human donor skin. We outline advantages and limitations of both models and discuss future perspectives in using HSEs.

AB - Human skin equivalents (HSEs) are three-dimensional skin organ culture models raised in vitro. This review gives an overview of common techniques for setting up HSEs. The HSE consists of an artificial dermis and epidermis. 3T3-J2 murine fibroblasts, purchased human fibroblasts or freshly isolated and cultured fibroblasts, together with other components, for example, collagen type I, are used to build the scaffold. Freshly isolated and cultured keratinocytes are seeded on top. It is possible to add other cell types, for example, melanocytes, to the HSE—depending on the research question. After several days and further steps, the 3D skin can be harvested. Additionally, we show possible markers and techniques for evaluation of artificial skin. Furthermore, we provide a comparison of HSEs to human skin organ culture, a model which employs human donor skin. We outline advantages and limitations of both models and discuss future perspectives in using HSEs.

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Requisite instruments for the establishment of three-dimensional epidermal human skin equivalents—A methods review

Abstract

Fördermittel

UN SDGs

Strategische Forschungsbereiche und Zentren

DFG-Fachsystematik

Dokumente

Weitere Links

Fingerprint

Zitieren