Abstract
Rat anterior pituitary quarters were incubated in vitro and the release of dynorphin A1-13-like immunoreactivity (Dyn A1-13-IR) into the incubation medium was studied. An elevation of the potassium concentration of the incubation medium to 40 mmol/l increased the release of Dyn A1-13-IR in a calcium-dependent manner. Addition of luteinizing hormone-releasing hormone (LHRH) stimulated the release of Dyn A1-13-IR in a dose-dependent manner. The LHRH-induced release of Dyn A1-13-IR was almost completely prevented by omission of calcium ions from the incubation medium or by addition of the LHRH-antagonist d-pGlu1, d-Phe2, d-Trp3,6-LHRH. This is the first demonstration that dynorphin-like immunoreactivity can be released from the rat adenohypophysis through calcium-dependent mechanisms by both high potassium concentration and receptor-mediated stimulation.
Originalsprache | Englisch |
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Zeitschrift | Naunyn-Schmiedebergs Archives of Pharmacology |
Jahrgang | 329 |
Ausgabenummer | 1 |
Seiten (von - bis) | 94-96 |
Seitenumfang | 3 |
ISSN | 0028-1298 |
DOIs | |
Publikationsstatus | Veröffentlicht - 03.1985 |
Strategische Forschungsbereiche und Zentren
- Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)