Rapid exchange of large numbers of donor- and host leukocytes after human liver transplantation

Catharina Heerwagen; Martin Schuster; Albrecht Bornscheurer; Lars Pape; Erich Kirchner; Hans Juergen Schlitt; Birgit Luettig; J. Westermann

doi:10.1007/s001470100323

Rapid exchange of large numbers of donor- and host leukocytes after human liver transplantation

Catharina Heerwagen, Martin Schuster, Albrecht Bornscheurer, Lars Pape, Erich Kirchner, Hans Juergen Schlitt, Birgit Luettig, J. Westermann^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Anatomie

Medizinische Hochschule Hannover

8 Zitate (Scopus)

Abstract

After liver transplantation, the release of donor leukocytes into the host and the uptake of host leukocytes by the graft is one of the earliest immunologic interactions between donor and host. Using three-color flow cytometry, these interactions were investigated in eight patients from 5 min-24 h after receiving HLA unmatched liver grafts. Five minutes after reperfusion, 5.0% ± 1.4% of all blood leukocytes in the host were of donor origin, decreasing to 1.1% ± 0.8% after 24 h. Donor granulocytes preferentially disappeared from the host circulation, whereas no differences were found between NK-cells and various B- and T cell subpopulations. Furthermore, host granulocytes were preferentially retained in the donor liver. Thus, despite extensive pre-operative perfusion, more than 10⁹ donor leukocytes quickly leave the liver graft while host granulocytes preferentially accumulate there. A better understanding of the molecular mechanisms mediating these early interactions might help to develop new strategies for diagnosis and therapy of liver graft rejection.

Originalsprache	Englisch
Zeitschrift	Transplant International
Jahrgang	14
Ausgabenummer	4
Seiten (von - bis)	240-247
Seitenumfang	8
ISSN	0934-0874
DOIs	https://doi.org/10.1007/s001470100323
Publikationsstatus	Veröffentlicht - 2001

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Acknowledgements We are indebted to Mrs. Johanna Schuler for her skillful technical assistance, to K.-H. Mahr for clinical support, and to R. Pabst for helpful suggestions regarding the manuscript. We would like to thank the nursing staff of the department of anesthesiology, the members of the RTransplantationsbüro Hannover“ and the transplant surgeons of the Medical School Hannover for their cooperation. This study was supported by the Deutsche For-schungsgemeinschaft (We 1175/4–3).

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Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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10.1007/s001470100323

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@article{c3ee6b1af6bb4a078ca14c5a8f06de61,

title = "Rapid exchange of large numbers of donor- and host leukocytes after human liver transplantation",

abstract = "After liver transplantation, the release of donor leukocytes into the host and the uptake of host leukocytes by the graft is one of the earliest immunologic interactions between donor and host. Using three-color flow cytometry, these interactions were investigated in eight patients from 5 min-24 h after receiving HLA unmatched liver grafts. Five minutes after reperfusion, 5.0\% ± 1.4\% of all blood leukocytes in the host were of donor origin, decreasing to 1.1\% ± 0.8\% after 24 h. Donor granulocytes preferentially disappeared from the host circulation, whereas no differences were found between NK-cells and various B- and T cell subpopulations. Furthermore, host granulocytes were preferentially retained in the donor liver. Thus, despite extensive pre-operative perfusion, more than 109 donor leukocytes quickly leave the liver graft while host granulocytes preferentially accumulate there. A better understanding of the molecular mechanisms mediating these early interactions might help to develop new strategies for diagnosis and therapy of liver graft rejection.",

author = "Catharina Heerwagen and Martin Schuster and Albrecht Bornscheurer and Lars Pape and Erich Kirchner and Schlitt, \{Hans Juergen\} and Birgit Luettig and J. Westermann",

note = "Funding Information: Acknowledgements We are indebted to Mrs. Johanna Schuler for her skillful technical assistance, to K.-H. Mahr for clinical support, and to R. Pabst for helpful suggestions regarding the manuscript. We would like to thank the nursing staff of the department of anesthesiology, the members of the RTransplantationsb{\"u}ro Hannover“ and the transplant surgeons of the Medical School Hannover for their cooperation. This study was supported by the Deutsche For-schungsgemeinschaft (We 1175/4–3). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "2001",

doi = "10.1007/s001470100323",

language = "English",

volume = "14",

pages = "240--247",

journal = "Transplant International",

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T1 - Rapid exchange of large numbers of donor- and host leukocytes after human liver transplantation

AU - Heerwagen, Catharina

AU - Schuster, Martin

AU - Bornscheurer, Albrecht

AU - Pape, Lars

AU - Kirchner, Erich

AU - Schlitt, Hans Juergen

AU - Luettig, Birgit

AU - Westermann, J.

N1 - Funding Information: Acknowledgements We are indebted to Mrs. Johanna Schuler for her skillful technical assistance, to K.-H. Mahr for clinical support, and to R. Pabst for helpful suggestions regarding the manuscript. We would like to thank the nursing staff of the department of anesthesiology, the members of the RTransplantationsbüro Hannover“ and the transplant surgeons of the Medical School Hannover for their cooperation. This study was supported by the Deutsche For-schungsgemeinschaft (We 1175/4–3). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 2001

Y1 - 2001

N2 - After liver transplantation, the release of donor leukocytes into the host and the uptake of host leukocytes by the graft is one of the earliest immunologic interactions between donor and host. Using three-color flow cytometry, these interactions were investigated in eight patients from 5 min-24 h after receiving HLA unmatched liver grafts. Five minutes after reperfusion, 5.0% ± 1.4% of all blood leukocytes in the host were of donor origin, decreasing to 1.1% ± 0.8% after 24 h. Donor granulocytes preferentially disappeared from the host circulation, whereas no differences were found between NK-cells and various B- and T cell subpopulations. Furthermore, host granulocytes were preferentially retained in the donor liver. Thus, despite extensive pre-operative perfusion, more than 109 donor leukocytes quickly leave the liver graft while host granulocytes preferentially accumulate there. A better understanding of the molecular mechanisms mediating these early interactions might help to develop new strategies for diagnosis and therapy of liver graft rejection.

AB - After liver transplantation, the release of donor leukocytes into the host and the uptake of host leukocytes by the graft is one of the earliest immunologic interactions between donor and host. Using three-color flow cytometry, these interactions were investigated in eight patients from 5 min-24 h after receiving HLA unmatched liver grafts. Five minutes after reperfusion, 5.0% ± 1.4% of all blood leukocytes in the host were of donor origin, decreasing to 1.1% ± 0.8% after 24 h. Donor granulocytes preferentially disappeared from the host circulation, whereas no differences were found between NK-cells and various B- and T cell subpopulations. Furthermore, host granulocytes were preferentially retained in the donor liver. Thus, despite extensive pre-operative perfusion, more than 109 donor leukocytes quickly leave the liver graft while host granulocytes preferentially accumulate there. A better understanding of the molecular mechanisms mediating these early interactions might help to develop new strategies for diagnosis and therapy of liver graft rejection.

UR - https://www.scopus.com/pages/publications/0034845542

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DO - 10.1007/s001470100323

M3 - Journal articles

C2 - 11512057

AN - SCOPUS:0034845542

SN - 0934-0874

VL - 14

SP - 240

EP - 247

JO - Transplant International

JF - Transplant International

IS - 4

ER -

Rapid exchange of large numbers of donor- and host leukocytes after human liver transplantation

Abstract

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