Quantifying Telomere Lengths of Human Individual Chromosome Arms by Centromere-Calibrated Fluorescence in Situ Hybridization and Digital Imaging

Sven Perner, Silke Brüderlein, Cornelia Hasel, Irena Waibel, Alexandra Holdenried, Neslisah Ciloglu, Heiko Chopurian, Kirsten Vang Nielsen, Andreas Plesch, Josef Högel, Peter Möller*

*Korrespondierende/r Autor/-in für diese Arbeit
75 Zitate (Scopus)

Abstract

Telomere length analysis has aroused considerable interest in biology and oncology. However, most published data are pan-genomic Southern-blot-based estimates. We developed T/C-FISH (telomere/centromere-FISH), allowing precise measurement of individual telomeres at every single chromosome arm. Metaphase preparations are co-hybridized with peptide nucleic acid probes for telomeric sequences and the chromosome 2 centromere serving as internal reference. Metaphase images are captured and karyotyped using dedicated software. A software module determines the absolute integrated fluorescence intensities of the p- and q-telomeres of each chromosome and the reference signal. Normalized data are derived by calculating the ratio of absolute telomere and reference signal intensities, and descriptive statistics are calculated. T/C-FISH detects even small differences in telomere length. Using T/C-FISH we have discovered an epigenetic process occurring in the human male postzygote or early embryo: in umbilical cord blood lymphocytes, telomeres on male Xqs are around 1100 bp shorter than female Xqs.

OriginalspracheEnglisch
ZeitschriftAmerican Journal of Pathology
Jahrgang163
Ausgabenummer5
Seiten (von - bis)1751-1756
Seitenumfang6
ISSN0002-9440
DOIs
PublikationsstatusVeröffentlicht - 11.2003

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