Production of coronavirus nonstructural proteins in soluble form for crystallization

Yvonne Piotrowski; Rajesh Ponnusamy; Stephanie Glaser; Anniken Daabach; Ralf Moll; Rolf Hilgenfeld

doi:10.1007/978-1-59745-181-9_13

Production of coronavirus nonstructural proteins in soluble form for crystallization

Yvonne Piotrowski^*, Rajesh Ponnusamy, Stephanie Glaser, Anniken Daabach, Ralf Moll, Rolf Hilgenfeld

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Biochemie

1 Zitat (Scopus)

Abstract

For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities.

Originalsprache	Englisch
Titel	SARS- and Other Coronaviruses : Laboratory Protocols
Seitenumfang	21
Herausgeber (Verlag)	Humana Press Inc.
Erscheinungsdatum	01.01.2008
Seiten	139-159
ISBN (Print)	9781588298676
DOIs	https://doi.org/10.1007/978-1-59745-181-9_13
Publikationsstatus	Veröffentlicht - 01.01.2008

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

Coronavirus-Bezug

Forschung zu SARS-CoV-2 / COVID-19

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

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10.1007/978-1-59745-181-9_13

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Production of coronavirus nonstructural proteins in soluble form for crystallization. / Piotrowski, Yvonne; Ponnusamy, Rajesh; Glaser, Stephanie et al.
SARS- and Other Coronaviruses: Laboratory Protocols. Humana Press Inc., 2008. S. 139-159 (Methods in Molecular Biology; Band 454).

Publikation: Kapitel in Büchern/Berichten/Konferenzbänden › Kapitel › Begutachtung

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AU - Piotrowski, Yvonne

AU - Ponnusamy, Rajesh

AU - Glaser, Stephanie

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AU - Moll, Ralf

AU - Hilgenfeld, Rolf

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Production of coronavirus nonstructural proteins in soluble form for crystallization

Abstract

Strategische Forschungsbereiche und Zentren

Coronavirus-Bezug

UN SDGs

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Andere Dateien und Links

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