Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

Shabnam Fayezi; Marefat Ghaffari Novin; Masoud Darabi; Mohsen Norouzian; Mohammad Nouri; Laya Farzadi; Maryam Darabi

doi:10.1177/1933719117698578

Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

Shabnam Fayezi, Marefat Ghaffari Novin^*, Masoud Darabi, Mohsen Norouzian, Mohammad Nouri, Laya Farzadi, Maryam Darabi

^*Korrespondierende/r Autor/-in für diese Arbeit

Klinik für Hämatologie und Onkologie

19 Zitate (Scopus)

Abstract

Stearoyl-coenzyme A desaturase 1 (SCD1) is a key enzyme in lipid metabolism and is expressed in cumulus cells. The objective of the present study was to evaluate the effect of SCD1 inhibition in human cumulus cells on triglyceride content, steroidogenesis, and oocyte in vitro maturation. Human cumulus cells were exposed to SCD1 inhibitor CAY10566 (SCDinhib) alone or in combination with oleic acid in primary culture. The SCDinhib markedly suppressed triglyceride accumulation (−47%, P =.01), aromatase gene expression (−36%, P =.02), and estradiol production (−49%, P =.01) even at a dose not affecting cell viability and apoptosis. Human immature oocytes at the germinal vesicle (GV) stage were cocultured with pretreated cumulus cells. The rate of oocytes reaching the metaphase II stage was significantly lower in coculture with SCDinhib-treated cumulus cells than with control cumulus cells (−18%, P <.01), which recovered by oleic acid supplementation. This finding on in vitro maturation rate was also reproducible with mouse GV oocytes. The results suggest that SCD1 activity is required for cumulus cell lipid storage and steroidogenesis. In addition, oocyte maturation is negatively affected by SCD1 inhibition in cumulus cells, possibly due to a deficient lipid-mediated paracrine support.

Originalsprache	Englisch
Zeitschrift	Reproductive Sciences
Jahrgang	25
Ausgabenummer	6
Seiten (von - bis)	844-853
Seitenumfang	10
ISSN	1933-7191
DOIs	https://doi.org/10.1177/1933719117698578
Publikationsstatus	Veröffentlicht - 01.06.2018

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The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work has been done as the first author’s requirement to complete her PhD thesis project by grants from the Office of the Vice President for Research at Shahid Beheshti University of Medical Sciences (#1393-1-91-13033) and Drug Applied Research Center at Tabriz University of Medical Sciences (#1393-7-28). The authors would like to acknowledge the Women?s Reproductive Health Research Center at Tabriz University of Medical Sciences for providing IVF laboratory facilities. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work has been done as the first author?s requirement to complete her PhD thesis project by grants from the Office of the Vice President for Research at Shahid Beheshti University of Medical Sciences (#1393-1-91-13033) and Drug Applied Research Center at Tabriz University of Medical Sciences (#1393-7-28).

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@article{f728cae3c1754db89adb5fcdfff03c04,

title = "Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation",

abstract = "Stearoyl-coenzyme A desaturase 1 (SCD1) is a key enzyme in lipid metabolism and is expressed in cumulus cells. The objective of the present study was to evaluate the effect of SCD1 inhibition in human cumulus cells on triglyceride content, steroidogenesis, and oocyte in vitro maturation. Human cumulus cells were exposed to SCD1 inhibitor CAY10566 (SCDinhib) alone or in combination with oleic acid in primary culture. The SCDinhib markedly suppressed triglyceride accumulation (−47\%, P =.01), aromatase gene expression (−36\%, P =.02), and estradiol production (−49\%, P =.01) even at a dose not affecting cell viability and apoptosis. Human immature oocytes at the germinal vesicle (GV) stage were cocultured with pretreated cumulus cells. The rate of oocytes reaching the metaphase II stage was significantly lower in coculture with SCDinhib-treated cumulus cells than with control cumulus cells (−18\%, P <.01), which recovered by oleic acid supplementation. This finding on in vitro maturation rate was also reproducible with mouse GV oocytes. The results suggest that SCD1 activity is required for cumulus cell lipid storage and steroidogenesis. In addition, oocyte maturation is negatively affected by SCD1 inhibition in cumulus cells, possibly due to a deficient lipid-mediated paracrine support.",

author = "Shabnam Fayezi and \{Ghaffari Novin\}, Marefat and Masoud Darabi and Mohsen Norouzian and Mohammad Nouri and Laya Farzadi and Maryam Darabi",

note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work has been done as the first author{\textquoteright}s requirement to complete her PhD thesis project by grants from the Office of the Vice President for Research at Shahid Beheshti University of Medical Sciences (\#1393-1-91-13033) and Drug Applied Research Center at Tabriz University of Medical Sciences (\#1393-7-28). Funding Information: The authors would like to acknowledge the Women?s Reproductive Health Research Center at Tabriz University of Medical Sciences for providing IVF laboratory facilities. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work has been done as the first author?s requirement to complete her PhD thesis project by grants from the Office of the Vice President for Research at Shahid Beheshti University of Medical Sciences (\#1393-1-91-13033) and Drug Applied Research Center at Tabriz University of Medical Sciences (\#1393-7-28). Publisher Copyright: {\textcopyright} 2017, {\textcopyright} The Author(s) 2017.",

year = "2018",

month = jun,

day = "1",

doi = "10.1177/1933719117698578",

language = "English",

volume = "25",

pages = "844--853",

journal = "Reproductive Sciences",

issn = "1933-7191",

publisher = "Springer Nature",

number = "6",

}

Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation. / Fayezi, Shabnam; Ghaffari Novin, Marefat; Darabi, Masoud et al.
in: Reproductive Sciences, Jahrgang 25, Nr. 6, 01.06.2018, S. 844-853.

Publikation: Beiträge in Fachzeitschriften › Zeitschriftenaufsätze › Forschung › Begutachtung

TY - JOUR

T1 - Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

AU - Fayezi, Shabnam

AU - Ghaffari Novin, Marefat

AU - Darabi, Masoud

AU - Norouzian, Mohsen

AU - Nouri, Mohammad

AU - Farzadi, Laya

AU - Darabi, Maryam

N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work has been done as the first author’s requirement to complete her PhD thesis project by grants from the Office of the Vice President for Research at Shahid Beheshti University of Medical Sciences (#1393-1-91-13033) and Drug Applied Research Center at Tabriz University of Medical Sciences (#1393-7-28). Funding Information: The authors would like to acknowledge the Women?s Reproductive Health Research Center at Tabriz University of Medical Sciences for providing IVF laboratory facilities. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work has been done as the first author?s requirement to complete her PhD thesis project by grants from the Office of the Vice President for Research at Shahid Beheshti University of Medical Sciences (#1393-1-91-13033) and Drug Applied Research Center at Tabriz University of Medical Sciences (#1393-7-28). Publisher Copyright: © 2017, © The Author(s) 2017.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Stearoyl-coenzyme A desaturase 1 (SCD1) is a key enzyme in lipid metabolism and is expressed in cumulus cells. The objective of the present study was to evaluate the effect of SCD1 inhibition in human cumulus cells on triglyceride content, steroidogenesis, and oocyte in vitro maturation. Human cumulus cells were exposed to SCD1 inhibitor CAY10566 (SCDinhib) alone or in combination with oleic acid in primary culture. The SCDinhib markedly suppressed triglyceride accumulation (−47%, P =.01), aromatase gene expression (−36%, P =.02), and estradiol production (−49%, P =.01) even at a dose not affecting cell viability and apoptosis. Human immature oocytes at the germinal vesicle (GV) stage were cocultured with pretreated cumulus cells. The rate of oocytes reaching the metaphase II stage was significantly lower in coculture with SCDinhib-treated cumulus cells than with control cumulus cells (−18%, P <.01), which recovered by oleic acid supplementation. This finding on in vitro maturation rate was also reproducible with mouse GV oocytes. The results suggest that SCD1 activity is required for cumulus cell lipid storage and steroidogenesis. In addition, oocyte maturation is negatively affected by SCD1 inhibition in cumulus cells, possibly due to a deficient lipid-mediated paracrine support.

AB - Stearoyl-coenzyme A desaturase 1 (SCD1) is a key enzyme in lipid metabolism and is expressed in cumulus cells. The objective of the present study was to evaluate the effect of SCD1 inhibition in human cumulus cells on triglyceride content, steroidogenesis, and oocyte in vitro maturation. Human cumulus cells were exposed to SCD1 inhibitor CAY10566 (SCDinhib) alone or in combination with oleic acid in primary culture. The SCDinhib markedly suppressed triglyceride accumulation (−47%, P =.01), aromatase gene expression (−36%, P =.02), and estradiol production (−49%, P =.01) even at a dose not affecting cell viability and apoptosis. Human immature oocytes at the germinal vesicle (GV) stage were cocultured with pretreated cumulus cells. The rate of oocytes reaching the metaphase II stage was significantly lower in coculture with SCDinhib-treated cumulus cells than with control cumulus cells (−18%, P <.01), which recovered by oleic acid supplementation. This finding on in vitro maturation rate was also reproducible with mouse GV oocytes. The results suggest that SCD1 activity is required for cumulus cell lipid storage and steroidogenesis. In addition, oocyte maturation is negatively affected by SCD1 inhibition in cumulus cells, possibly due to a deficient lipid-mediated paracrine support.

UR - https://www.scopus.com/pages/publications/85042589893

U2 - 10.1177/1933719117698578

DO - 10.1177/1933719117698578

M3 - Journal articles

C2 - 28345489

AN - SCOPUS:85042589893

SN - 1933-7191

VL - 25

SP - 844

EP - 853

JO - Reproductive Sciences

JF - Reproductive Sciences

IS - 6

ER -

Primary Culture of Human Cumulus Cells Requires Stearoyl-Coenzyme A Desaturase 1 Activity for Steroidogenesis and Enhancing Oocyte In Vitro Maturation

Abstract

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