Predicting the orientation of eukaryotic membrane-spanning proteins

E. Hartmann; T. A. Rapoport; H. F. Lodish

doi:10.1073/pnas.86.15.5786

Predicting the orientation of eukaryotic membrane-spanning proteins

E. Hartmann, T. A. Rapoport, H. F. Lodish

Institut für Biologie

Massachusetts Institute of Technology

450 Zitate (Scopus)

Abstract

We have developed a rule to predict the orientation of the first internal signal-anchor sequence in eukaryotic transmembrane proteins synthesized on the rough endoplasmic reticulum. The difference in the charges of the 15 residues flanking the first internal signal-anchor determine its orientation, with the more positive portion facing the cytosol. In proteins that span the membrane more than once, the orientation of all subsequent transmembrane segments would be determined by that of the most N-terminal one.

Originalsprache	Englisch
Zeitschrift	Proceedings of the National Academy of Sciences of the United States of America
Jahrgang	86
Ausgabenummer	15
Seiten (von - bis)	5786-5790
Seitenumfang	5
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.86.15.5786
Publikationsstatus	Veröffentlicht - 01.01.1989

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Predicting the orientation of eukaryotic membrane-spanning proteins

Abstract

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