Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells

Catrin Roolf; Christin Kretzschmar; Katrin Timmer; Anett Sekora; Gudrun Knübel; Hugo Murua Escobar; Georg Fuellen; Saleh M. Ibrahim; Markus Tiedge; Simone Baltrusch; Sarah Müller; Rüdiger Köhling; Christian Junghanss

doi:10.21873/invivo.10991

Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells

Catrin Roolf, Christin Kretzschmar, Katrin Timmer, Anett Sekora, Gudrun Knübel, Hugo Murua Escobar, Georg Fuellen, Saleh M. Ibrahim, Markus Tiedge, Simone Baltrusch, Sarah Müller, Rüdiger Köhling, Christian Junghanss^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Klinik für Dermatologie, Allergologie und Venerologie

Universität Rostock

1 Zitat (Scopus)

Abstract

Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.

Originalsprache	Englisch
Zeitschrift	In Vivo
Jahrgang	30
Ausgabenummer	6
Seiten (von - bis)	751-760
Seitenumfang	10
ISSN	0258-851X
DOIs	https://doi.org/10.21873/invivo.10991
Publikationsstatus	Veröffentlicht - 01.11.2016

Strategische Forschungsbereiche und Zentren

Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

UN SDGs

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10.21873/invivo.10991

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@article{dc0347baae2c47d2a2b6934a4c5b777e,

title = "Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells",

abstract = "Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.",

author = "Catrin Roolf and Christin Kretzschmar and Katrin Timmer and Anett Sekora and Gudrun Kn{\"u}bel and Escobar, {Hugo Murua} and Georg Fuellen and Ibrahim, {Saleh M.} and Markus Tiedge and Simone Baltrusch and Sarah M{\"u}ller and R{\"u}diger K{\"o}hling and Christian Junghanss",

year = "2016",

month = nov,

day = "1",

doi = "10.21873/invivo.10991",

language = "English",

volume = "30",

pages = "751--760",

journal = "In Vivo",

issn = "0258-851X",

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TY - JOUR

T1 - Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells

AU - Roolf, Catrin

AU - Kretzschmar, Christin

AU - Timmer, Katrin

AU - Sekora, Anett

AU - Knübel, Gudrun

AU - Escobar, Hugo Murua

AU - Fuellen, Georg

AU - Ibrahim, Saleh M.

AU - Tiedge, Markus

AU - Baltrusch, Simone

AU - Müller, Sarah

AU - Köhling, Rüdiger

AU - Junghanss, Christian

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.

AB - Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.

UR - http://www.scopus.com/inward/record.url?scp=84994205147&partnerID=8YFLogxK

U2 - 10.21873/invivo.10991

DO - 10.21873/invivo.10991

M3 - Journal articles

C2 - 27815458

AN - SCOPUS:84994205147

SN - 0258-851X

VL - 30

SP - 751

EP - 760

JO - In Vivo

JF - In Vivo

IS - 6

ER -

Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells

Abstract

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