Pathogenic Activation and Therapeutic Blockage of FcαR-Expressing Polymorphonuclear Leukocytes in IgA Pemphigus

Shirin Emtenani, Saeedeh Ghorbanalipoor, Sarah Mayer-Hain, Khalaf Kridin, Lars Komorowski, Christian Probst, Takashi Hashimoto, Hendri H. Pas, Kaja Męcińska-Jundziłł, Rafał Czajkowski, Andreas Recke, Cord Sunderkötter, Stefan W. Schneider, Jennifer E. Hundt, Detlef Zillikens, Enno Schmidt, Ralf J. Ludwig, Christoph M. Hammers*

*Korrespondierende/r Autor/-in für diese Arbeit

Abstract

Pathomechanisms in IgA pemphigus are assumed to rely on Fc-dependent cellular activation by antigen-specific IgA autoantibodies; however, models for the disease and more detailed pathophysiologic data are lacking. In this study, we aimed to establish in vitro models of disease for IgA pemphigus, allowing us to study the effects of the interaction of anti-keratinocyte IgA with cell surface FcαRs. Employing multiple in vitro assays, such as a skin cryosection assay and a human skin organ culture model, in this study, we present mechanistic data for the pathogenesis of IgA pemphigus, mediated by anti–desmoglein 3 IgA autoantibodies. Our results reveal that this disease is dependent on FcαR-mediated activation of leukocytes in the epidermis. Importantly, this cell-dependent pathology can be dose-dependently abrogated by peptide-mediated inhibition of FcαR:IgA-Fc interaction, as confirmed in an additional model for IgA-dependent disease, that is, IgA vasculitis. These data suggest that IgA pemphigus can be modeled in vitro and that IgA pemphigus and IgA vasculitis are FcαR-dependent disease entities that can be specifically targeted in these experimental systems.

OriginalspracheEnglisch
ZeitschriftJournal of Investigative Dermatology
Jahrgang141
Ausgabenummer12
Seiten (von - bis)2820-2828
Seitenumfang9
ISSN0022-202X
DOIs
PublikationsstatusVeröffentlicht - 12.2021

Strategische Forschungsbereiche und Zentren

  • Forschungsschwerpunkt: Infektion und Entzündung - Zentrum für Infektions- und Entzündungsforschung Lübeck (ZIEL)

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