Optical Microscopy and Spectroscopy of Single Molecules

Christian Hübner; Thomas Basché

doi:10.1002/3527607323.ch2

Optical Microscopy and Spectroscopy of Single Molecules

Christian Hübner^*, Thomas Basché

^*Korrespondierende/r Autor/-in für diese Arbeit

Institut für Physik

Johannes Gutenberg-Universität Mainz

Abstract

This chapter contains sections titled:

Introduction

Photophysical Principles of Single‐Molecule Fluorescence Detection

The Single Molecule as a Three‐Level System

Dipole–Dipole Coupled Oscillators

Weak Coupling

Strong Coupling

Experimental Techniques

Signal‐to‐Noise Considerations

Room‐Temperature Single‐Molecule Spectroscopy

Epifluorescence Microscopy

Total Internal Reflection (TIR) Microscopy

Scanning Confocal Optical Microscopy

Two‐Photon‐, 4π‐ and STED Microscopy

Scanning Near‐Field Optical Microscopy

Single‐Molecule Spectroscopy at Cryogenic Temperatures

The Laser System

Applications

Photon Antibunching

Photon Bunching

Electronic Coupling Between Molecules

Single Molecules as Antennas: Orientation

Originalsprache	Englisch
Titel	Photophysics of Molecular Materials: From Single Molecules to Single Crystals
Seitenumfang	43
Herausgeber (Verlag)	Wiley-VCH Verlag GmbH & Co. KGaA
Erscheinungsdatum	27.04.2006
Seiten	5-47
ISBN (Print)	9783527404568
DOIs	https://doi.org/10.1002/3527607323.ch2
Publikationsstatus	Veröffentlicht - 27.04.2006

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Optical Microscopy and Spectroscopy of Single Molecules

Abstract

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